A single-cell genome reveals diplonemid-like ancestry of kinetoplastid mitochondrial gene structure
| Autor: | David S. Milner, Raquel Rodríguez-Martínez, Alastair G. B. Simpson, Guy Leonard, Thomas A. Richards, Jeremy G. Wideman, Gordon Lax |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Mitochondrial DNA Euglenozoa Genomics 010603 evolutionary biology 01 natural sciences Genome General Biochemistry Genetics and Molecular Biology 03 medical and health sciences evolution genomics Gene 030304 developmental biology 0303 health sciences diplonemids Phylogenetic tree biology single-cell genomics fungi microbiology Articles biology.organism_classification kinetoplastids Sister group Evolutionary biology RNA editing mitochondrial genome Genome Mitochondrial Single-Cell Analysis General Agricultural and Biological Sciences Genome Protozoan Research Article |
| Zdroj: | Philosophical Transactions of the Royal Society B: Biological Sciences |
| Popis: | Euglenozoa comprises euglenids, kinetoplastids, and diplonemids, with each group exhibiting different and highly unusual mitochondrial genome organizations. Although they are sister groups, kinetoplastids and diplonemids have very distinct mitochondrial genome architectures, requiring widespread insertion/deletion RNA editing and extensive trans -splicing, respectively, in order to generate functional transcripts. The evolutionary history by which these differing processes arose remains unclear. Using single-cell genomics, followed by small sub unit ribosomal DNA and multigene phylogenies, we identified an isolated marine cell that branches on phylogenetic trees as a sister to known kinetoplastids. Analysis of single-cell amplified genomic material identified multiple mitochondrial genome contigs. These revealed a gene architecture resembling that of diplonemid mitochondria, with small fragments of genes encoded out of order and or on different contigs, indicating that these genes require extensive trans -splicing. Conversely, no requirement for kinetoplastid-like insertion/deletion RNA-editing was detected. Additionally, while we identified some proteins so far only found in kinetoplastids, we could not unequivocally identify mitochondrial RNA editing proteins. These data invite the hypothesis that extensive genome fragmentation and trans -splicing were the ancestral states for the kinetoplastid-diplonemid clade but were lost during the kinetoplastid radiation. This study demonstrates that single-cell approaches can successfully retrieve lineages that represent important new branches on the tree of life, and thus can illuminate major evolutionary and functional transitions in eukaryotes. This article is part of a discussion meeting issue ‘Single cell ecology’. |
| Databáze: | OpenAIRE |
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