Evaluation of a nanotechnology-based approach to induce gene-expression in human THP-1 macrophages under inflammatory conditions
Autor: | Bailey Hill, Candler Paige, E. Alfonso Romero-Sandoval, Laura Bernal, Abigail Alvarado-Vázquez, David Wilson Ferreira, Marina Caesar, Cristina Ulecia-Morón |
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Rok vydání: | 2017 |
Předmět: |
Lipopolysaccharides
0301 basic medicine Lipopolysaccharide CD14 Immunology Macrophage-activating factor Gene Expression Inflammation Biology Gene delivery Transfection Article Cell Line 03 medical and health sciences chemistry.chemical_compound Genes Reporter medicine Humans Nanotechnology Immunology and Allergy RNA Messenger Macrophage inflammatory protein Macrophages Gene Transfer Techniques Cell Differentiation Hematology Molecular biology Cell biology 030104 developmental biology chemistry Cytokines Nanoparticles Tumor necrosis factor alpha Inflammation Mediators medicine.symptom Mannose receptor |
Zdroj: | Immunobiology |
ISSN: | 0171-2985 |
DOI: | 10.1016/j.imbio.2016.08.010 |
Popis: | Macrophages orchestrate the initiation and resolution of inflammation by producing pro- and anti-inflammatory products. An imbalance in these mediators may originate from a deficient or excessive immune response. Therefore, macrophages are valid therapeutic targets to restore homeostasis under inflammatory conditions. We hypothesize that a specific mannosylated nanoparticle effectively induces gene expression in human macrophages under inflammatory conditions without undesirable immunogenic responses. THP-1 macrophages were challenged with lipopolysaccharide (LPS, 5 μg/mL). Polyethylenimine (PEI) nanoparticles grafted with a mannose receptor ligand (Man-PEI) were used as a gene delivery method. Nanoparticle toxicity, Man-PEI cellular uptake rate and gene induction efficiency (GFP, CD14 or CD68) were studied. Potential immunogenic responses were evaluated by measuring the production of tumor necrosis factor-alpha (TNF-α), Interleukin (IL)-6 and IL-10. Man-PEI did not produce cytotoxicity, and it was effectively up-taken by THP-1 macrophages (69%). This approach produced a significant expression of GFP (mRNA and protein), CD14 and CD68 (mRNA), and transiently and mildly reduced IL-6 and IL-10 levels in LPS-challenged macrophages. Our results indicate that Man-PEI is suitable for inducing an efficient gene overexpression in human macrophages under inflammatory conditions with limited immunogenic responses. Our promising results set the foundation to test this technology to induce functional anti-inflammatory genes. |
Databáze: | OpenAIRE |
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