Optimization of enzymatic synthesis of l-arabinose ferulate catalyzed by feruloyl esterases from Myceliophthora thermophila in detergentless microemulsions and assessment of its antioxidant and cytotoxicity activities
| Autor: | Paul Christakopoulos, Marianna Ralli, Io Antonopoulou, Peter Jütten, Alexander Piechot, Adamantia Papadopoulou, Laura Iancu, Dimitris Kletsas, Ulrika Rova, Gabriella Cerullo, Vincenza Faraco |
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| Přispěvatelé: | Antonopoulou, Io, Papadopoulou, Adamantia, Iancu, Laura, Cerullo, Gabriella, Ralli, Marianna, Jütten, Peter, Piechot, Alexander, Faraco, Vincenza, Kletsas, Dimitri, Rova, Ulrika, Christakopoulos, Paul |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant Cytotoxicity medicine.medical_treatment Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Catalysis 03 medical and health sciences C1 L-Arabinose 010608 biotechnology medicine Microemulsion L-arabinose ferulate biology DCFH-DA Transesterification Enzymatic synthesis biology.organism_classification Activity 030104 developmental biology Feruloyl esterase Myceliophthora thermophila DPPH |
| Zdroj: | Process Biochemistry. 65:100-108 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2017.11.009 |
| Popis: | The feruloyl esterases FaeA1, FaeA2, FaeB1, FaeB2 from Myceliophthora thermophila C1 and MtFae1a from M. thermophila ATCC 42464 were used as biocatalysts for the transesterification of vinyl ferulate (VFA) with Larabinose in detergentless microemulsions. The effect of parameters such as the microemulsion composition, the substrate concentration, the enzyme load, the pH, the temperature and the agitation was investigated. FaeA1 offered the highest transesterification yield (52.2 ± 4.3%) after 8 h of incubation at 50 °C using 80 mM VFA, 55 mM L-arabinose and 0.02 mg FAE mL−1 in a mixture comprising of 19.8: 74.7: 5.5 v/v/v n-hexane: t-butanol: 100 mM MOPS-NaOH pH 8.0. The ability of L-arabinose ferulate (AFA) to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals was significant (IC50 386.5 μM). AFA was not cytotoxic even at high concentrations (1 mM) however was found to be pro-oxidant at concentrations higher than 20 μM when the antioxidant activity was determined with the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay in human skin fibroblasts. |
| Databáze: | OpenAIRE |
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