Thymbra capitata essential oil has a significant antimicrobial activity against methicillin-resistant Staphylococcus aureus pre-formed biofilms
| Autor: | L. Almeida, N. Lopes, V. Gaio, C. Cavaleiro, L. Salgueiro, V. Silva, P. Poeta, N. Cerca |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Science & Technology cell culturability cellular activity minimum lethal concentration (MLC) Microbial Sensitivity Tests MRSA XTT biochemical phenomena metabolism and nutrition minimum inhibitory concentration Applied Microbiology and Biotechnology Anti-Bacterial Agents biofilm biomass minimum inhibitory concentration (MIC) Biofilms EO minimum lethal concentration Oils Volatile |
| Popis: | Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant opportunistic pathogen with a great ability to form biofilms. Herein, the antimicrobial potential of Thymbra capitata essential oil (EO) against MRSA biofilms was investigated. The determination of the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the T. capitata EO was first investigated on a group of clinical isolates from septicemias, diabetic foot ulcers and osteomyelitis. Biofilms were incubated with the EO at the MLC and its anti-biofilm potential was investigated. A strong antimicrobial activity was observed, with MIC and MLC values between 0.32 and 0.64 mg L-1. However, the concentration of EO necessary for the eradication of planktonic cells was insufficient to significantly reduce the biofilm biomass of some isolates. Nevertheless, cell culturability and overall cellular metabolism was strongly reduced in all biofilms tested, only when the EO was tested. Contrary to the tested antibiotics, T. capitata EO showed a significant antimicrobial activity against MRSA biofilms, by reducing cellular metabolism and cellular culturability. NC acknowledges the Portuguese Foundation for Science and Technology (FCT), with the strategic funding of the unit (UIDB/04469/2020). PP also acknowledges the funding received from projects with reference NORTE-01-0145-FEDER-030101 (financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020), PTDC/SAU-INF/30101/2017 and UIDB/CVT/00772/2020 (financed by FCT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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