Ovotransferrin is a matrix protein of the hen eggshell membranes and basal calcified layer
| Autor: | Yves Nys, Joël Gautron, Juan Manuel García-Ruiz, M. Panheleux, T. Boldicke, Maxwell T. Hincke |
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| Přispěvatelé: | Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Oviducts
Biochemistry Egg Shell chemistry.chemical_compound Tissue Distribution Orthopedics and Sports Medicine Eggshell Tubular gland ComputingMilieux_MISCELLANEOUS Calcite Extracellular Matrix Proteins 0303 health sciences Crystallography [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology CRISTALOGRAPHIE 04 agricultural and veterinary sciences Body Fluids medicine.anatomical_structure Membrane Oviduct Female Crystallization Conalbumin Biology Calcium Carbonate 03 medical and health sciences Rheumatology medicine Animals Amino Acid Sequence RNA Messenger Molecular Biology 030304 developmental biology Membranes urogenital system Egg Proteins Uterus 0402 animal and dairy science Cell Biology Ovotransferrin 040201 dairy & animal science Calcium carbonate chemistry Polyclonal antibodies biology.protein Biophysics Calcium Chickens [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | Connective Tissue Research Connective Tissue Research, Taylor & Francis, 2001, 42 (4), pp.255-267 |
| ISSN: | 0300-8207 |
| Popis: | The eggshell is an highly ordered structure deposited in the distal oviduct and composed of calcium carbonate and an organic matrix which is believed to influence its fabric. We have identified ovotransferrin as an 80 kDa matrix protein observed at high concentration in the uterine fluid at the initial stage of shell mineralization, by N-terminal sequencing and western blotting using monoclonal and polyclonal antibodies. It is present in extracts from demineralized eggshell and was localized by immunofluorescence in the eggshell membranes and mammillae, which are the sites of calcite nucleation. Northern blotting and RT-PCR demonstrated that ovotransferrin message was expressed in the proximal oviduct (magnum and white isthmus), and at a lower magnitude in the distal oviduct (red isthmus and uterus). Ovotransferrin was revealed by immunofluorescence in the tubular gland cells of the uterus. Calcium carbonate crystals grown in vitro in the presence of purified ovotransferrin showed large modifications of the calcite morphology. These observations and its presence in eggshell and membranes suggest a dual role for ovotransferrin, as a protein influencing nucleation and growth of calcite crystals and as a bacteriostatic filter to reinforce its inhibition of Salmonella growth in egg albumen. |
| Databáze: | OpenAIRE |
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