Decreased Th17 and Th1 cells in the peripheral blood of patients with early non-radiographic axial spondyloarthritis: a marker of disease activity in HLA-B27⁺ patients
| Autor: | Amaya Puig-Kröger, Irene Arroyo-Villa, Maria-Eugenia Miranda-Carus, Emilio Martín-Mola, María-Belén Bautista-Caro, Eugenio de Miguel, Diana Peiteado, Concepción Castillo-Gallego |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male medicine.medical_specialty Adolescent chemical and pharmacologic phenomena Enzyme-Linked Immunosorbent Assay Severity of Illness Index Interleukin 22 Young Adult Rheumatology Internal medicine Spondylarthritis medicine Humans Pharmacology (medical) Interleukin 5 BASDAI Interleukin 4 HLA-B27 Antigen business.industry Tumor Necrosis Factor-alpha Interleukin hemic and immune systems Middle Aged Th1 Cells Flow Cytometry Interleukin-10 Interleukin 10 Endocrinology Phenotype Case-Control Studies Quality of Life Cytokines Th17 Cells Female Interleukin 17 business Cytometry Biomarkers |
| Zdroj: | Rheumatology (Oxford, England). 52(2) |
| ISSN: | 1462-0332 |
| Popis: | OBJECTIVE To examine the frequency and phenotype of Th17 cells in the peripheral blood of patients with early non-radiographic axial SpA (early nrSpA). METHODS CD4(+) T cells were isolated from the peripheral blood of 30 early nrSpA patients, 11 AS patients and 41 age- and sex-matched healthy controls by Ficoll-Hypaque gradient and magnetic negative selection. After polyclonal stimulation, the frequency of Th17 and Th1 cells and of cells producing TNF-α or IL-10 was determined by cytometry and concentrations of IL-17, IL-22, IFN-γ, TNF-α, IL-10 and IL-4 were measured by ELISA. RESULTS Early nrSpA but not AS patients demonstrated a significantly lower percentage of circulating Th17, Th1 and Th17/Th1 cells, together with lower CD4-derived IL-17 and IFN-γ secretion, as compared with controls. In contrast, the percentage of circulating cells producing IL-10 or TNF-α, and the secretion of CD4-derived IL-10, TNF-α, IL-22 and IL-4 in early nrSpA were not different from controls. All Th17 cells were CD45RO(+)CD45RA(-) and CCR6(+). The frequency of circulating Th17, Th1 and Th17/Th1 was negatively correlated with BASDAI, BASFI, ASDAS-CRP, ASDAS-ESR, AS quality of life (ASQOL) and patient's global assessment in HLA-B27(+) but not in HLA-B27(-) early nrSpA patients. A positive correlation between circulating Th17 cells and BASDAI was observed in AS. CONCLUSION A decreased percentage of Th17, Th1 and Th17/Th1 cells is apparent in peripheral blood CD4(+) T cells from early nrSpA. Th17, Th1 and Th17/Th1 cell numbers are related to disease activity indices in HLA-B27(+), but not in HLA-B27(-), early nrSpA patients. |
| Databáze: | OpenAIRE |
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