The Role of HER2 in Self-Renewal, Invasion, and Tumorigenicity of Gastric Cancer Stem Cells
| Autor: | Zheng-Hao Lu, Zong-Guang Zhou, Yi-Gao Wang, Pei-Xian Hou, Xiao-Long Chen, Jiankun Hu, Yu-Xin Liu, Li-Fei Sun, Xianming Mo, Kun Yang, Wei-Han Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
cancer stem cells
0301 basic medicine Cancer Research invasiveness Biology lcsh:RC254-282 03 medical and health sciences 0302 clinical medicine Cancer stem cell In vivo HER2 medicine skin and connective tissue diseases neoplasms PI3K/AKT/mTOR pathway Original Research Gene knockdown gastric cancer Cancer Transfection lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer cell Cancer research tumorigenicity Signal transduction |
| Zdroj: | Frontiers in Oncology Frontiers in Oncology, Vol 10 (2020) |
| ISSN: | 2234-943X |
| DOI: | 10.3389/fonc.2020.01608 |
| Popis: | Background: Deregulation of HER2 expression could affect the biological characteristics of gastric cancer cells and treatment option for gastric cancer patients. This research aims to investigate the impact of HER2 on biological characteristics of gastric cancer stem cells (GCSCs) and prognosis of gastric cancer patients. Methods: HER2 knockdown in GCSCs were constructed by lentivirus transfection. Changes of proliferation, self-renewal, invasion, migration, colony formation, and tumorigenicity of GCSCs were examined. The changes of gene expressions after HER2 interference in GCSCs were detected by gene microarray. The impact of concentrations of serum HER2 and expression of HER2 in tumor tissues on survival of 213 gastric cancer patients were also analyzed. Results: The down-regulation of HER2 decreased the self-renewal, colony formation, migration, invasion, proliferation, chemotherapy resistance of GCSCs. However, the tumorigenicity of GCSCs in vivo was increased after down-regulation of HER2. The results of gene microarray showed that HER2 gene might regulate the signal transduction of mTOR, Jak-STAT and other signal pathways, and affect the biological characteristics of GCSCs. Furthermore, survival analyses indicated that patients with high concentration of HER2 in serum had a favorable overall survival. However, there was no significant correlation between expression of HER2 in tumor tissue and overall survival. Conclusions: Interference of HER2 in GCSCs decreased the capacity of self-renewal, proliferation, colony formation, chemotherapy resistance, invasion and migration, but might increase the tumorigenicity in vivo. Patients with high concentration of HER2 in serum seemed to have a favorable prognosis. Key words: Gastric cancer1; Cancer stem cells2; HER23; Tumorigenicity4 |
| Databáze: | OpenAIRE |
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