Multimodal Tandem Mass Spectrometry Techniques for the Analysis of Phosphopeptides

Autor:	Johanna Paris, Alina Theisen, Bryan P. Marzullo, Anisha Haris, Tomos E. Morgan, Mark P. Barrow, John O’Hara, Peter B. O’Connor
Rok vydání:	2022
Předmět:	Phosphopeptides Fourier Analysis Tandem Mass Spectrometry Structural Biology Electrons QD Cyclotrons Spectroscopy
Zdroj:	Journal of the American Society for Mass Spectrometry. 33:1126-1133
ISSN:	1879-1123 1044-0305
DOI:	10.1021/jasms.1c00353
Popis:	Collisionally activated dissociation (CAD), infrared multiphoton dissociation (IRMPD), ultraviolet photodissociation (UVPD), electron capture dissociation and electron detachment dissociation (EDD) experiments were conducted on a set of phosphopeptides, in a Fourier transform ion cyclotron resonance mass spectrometer. The fragmentation patterns were compared and varied according to the fragmentation mechanisms and the composition of the peptides. CAD and IRMPD produced similar fragmentation profiles of the phosphopeptides, while UVPD produced a large number of complementary fragments. Electron-based dissociation techniques displayed lower fragmentation efficiencies, despite retaining the labile phosphate group, and drastically different fragmentation profiles. EDD produced complex spectra whose interpretation proved challenging.\ud \ud
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5d59a55902d800ab16a07e17dd6f8dd https://doi.org/10.1021/jasms.1c00353 Zobrazit plný text záznamu