Evaluation of Cytotoxicity and Genotoxicity ofAcacia aromaLeaf Extracts
| Autor: | C. Sabini, A. Sosa, A. L. Laciar, C. M. Mattana, Liliana Sabini, Franco Matías Escobar, M. A. Cangiano, L. E. Alcaráz |
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| Rok vydání: | 2014 |
| Předmět: |
Cytotoxicity
lcsh:Medicine medicine.disease_cause lcsh:Technology purl.org/becyt/ford/1 [https] Toxicology chemistry.chemical_compound Antiseptic Chlorocebus aethiops Medicine Food science lcsh:Science General Environmental Science Gel electrophoresis Cell Death biology Acacia General Medicine CIENCIAS NATURALES Y EXACTAS Research Article Acacia aroma Adult Neutral red Article Subject Cell Survival medicine.drug_class DNA damage Otras Ciencias Biológicas General Biochemistry Genetics and Molecular Biology Extracts Ciencias Biológicas Animals Humans purl.org/becyt/ford/1.6 [https] Cell Shape Vero Cells Aroma Plant Extracts lcsh:T business.industry lcsh:R biology.organism_classification Plant Leaves Comet assay chemistry lcsh:Q Genotoxicity business DNA Damage Mutagens |
| Zdroj: | The Scientific World Journal, Vol 2014 (2014) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET The Scientific World Journal |
| ISSN: | 1537-744X 2356-6140 |
| Popis: | Acacia aroma, native plant from San Luis, Argentina, is commonly used as antiseptic and for healing of wounds. The present study was conducted to investigate the in vitro cytotoxicity and genotoxicity of hot aqueous extract (HAE) and ethanolic extract (EE) of A. aroma. The cytotoxic activity was assayed by neutral red uptake assay on Vero cell. Cell treatment with a range from 100 to 5000 g/mL of HAE and EE showed that 500 g/mL and 100 g/mL were the maximum noncytotoxic concentrations, respectively. The CC50 was 658 g/mL for EE and 1020 g/mL for HAE. The genotoxicity was tested by the single-cell gel electrophoresis comet assay. The results obtained in the evaluation of DNA cellular damage exposed to varied concentrations of the HAE showed no significant genotoxic effect at range of 1–20 mg/mL. The EE at 20 mg/mL showed moderate genotoxic effect related to the increase of the DNA percentage contained in tail of the comet; DNA was classified in category 2. At concentrations below 5 mg/mL, the results of cytotoxicity and genotoxicity of aqueous and ethanolic extracts of Acacia aroma guarantee the safety at cell and genomic level. However further studies are needed for longer periods including animal models to confirm the findings. Fil: Mattana, Claudia Maricel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Cangiano, Maria de Los Angeles. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alcaraz, María Luciana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sosa, A.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sabini, C.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina Fil: Sabini, Liliana Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina Fil: Laciar, Analia Liliana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina |
| Databáze: | OpenAIRE |
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