A Chinese herbal decoction can increase the intracellular Ca2+ concentration and CatSper1 expression in mouse sperm tails
| Autor: | Hai-Xiang Hu, Yong-Zheng Jiao, Shao-Bo Luo, Jin-Ming Jia, Jia-Chen Dong, Wei-Guo Ma |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
endocrine system Cancer Research medicine.medical_treatment Intraperitoneal injection Decoction Biology Biochemistry Calcium in biology Andrology Mice Genetics medicine Animals Molecular Biology reproductive and urinary physiology Sperm motility Calcium metabolism urogenital system Spermatozoa Sperm Molecular biology Gene Expression Regulation Oncology Apoptosis Sperm Tail Sperm Motility Molecular Medicine Calcium Calcium Channels Intracellular Drugs Chinese Herbal |
| Zdroj: | Molecular Medicine Reports. 7:195-200 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2012.1118 |
| Popis: | The aim of this study was to investigate the impact of a Chinese herbal decoction on the intracellular calcium (Ca2+) concentration of sperm and the expression of the cation channel 1 of sperm (CatSper1), which is a calcium-channel protein specific to sperm tail, in a murine model of asthenospermia induced with cyclophosphamide. After 34 days of intragastric administration of Chinese herb decoction to the murine model used, routine analyses of the mouse sperm were conducted, the intracellular Ca2+ concentration of the sperm tails was measured using flow cytometry, and the expression of CatSper1 protein was detected using reverse transcription‑polymerase chain reaction (RT-PCR). Sperm concentration, percentage of grade A and B sperm (i.e., sperm activity) and percentage of grade A, B and C sperm (i.e., overall sperm motility) of the model group mice (MG) were markedly lower compared to the control murine group (CG) (one-way ANOVA, P |
| Databáze: | OpenAIRE |
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