Genomic imprinting defect in Zfp57 mutant iPS cell lines
| Autor: | Lijuan Xiao, Carol M. McDonald, Christoph Schaniel, Xiajun Li, Lizhi Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genotype Induced Pluripotent Stem Cells Mutant Mutagenesis (molecular biology technique) Biology Article Cell Line Genomic Imprinting Mice 03 medical and health sciences Animals Induced pluripotent stem cell lcsh:QH301-705.5 Embryoid Bodies Medicine(all) Genetics Mice Inbred ICR Zygote Cell Biology General Medicine DNA Methylation Fibroblasts Embryonic stem cell DNA-Binding Proteins 030104 developmental biology lcsh:Biology (General) Microscopy Fluorescence Mutagenesis DNA methylation Genomic imprinting Reprogramming Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 16, Iss 2, Pp 259-263 (2016) |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2016.01.018 |
| Popis: | ZFP57 maintains genomic imprinting in mouse embryos and ES cells. To test its roles during iPS reprogramming, we derived iPS clones by utilizing retroviral infection to express reprogramming factors in mouse MEF cells. After analyzing four imprinted regions, we found that parentally derived DNA methylation imprint was largely maintained in the iPS clones with Zfp57 but missing in those without maternal or zygotic Zfp57. Intriguingly, DNA methylation imprint was lost at the Peg1 and Peg3 but retained at the Snrpn and Dlk1-Dio3 imprinted regions in the iPS clones without zygotic Zfp57. This finding will be pursued in future studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|