Platelet function in baboons and humans - A comparative study of whole blood using impedance platelet aggregometry (Multiplate®)

Autor: Heinz Redl, Christoph J. Schlimp, Martin Ponschab, Herbert Schöchl, Stefan Heitmeier, Martijn van Griensven, Andreas Calatzis, Volker Laux, Soheyl Bahrami
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Male
Peptide Fragments/metabolism
Platelet Aggregation
030204 cardiovascular system & hematology
ACTIVATION
chemistry.chemical_compound
0302 clinical medicine
Baboons
INFECTION
Electric Impedance
Platelet
Whole blood
Adenosine Diphosphate/metabolism
biology
Hematology
Receptors
Thrombin/metabolism
Adenosine Diphosphate
RECEPTORS
Coagulation
030220 oncology & carcinogenesis
Blood Platelets/cytology
Collagen
medicine.symptom
Receptor
Blood Platelets
Platelets
medicine.medical_specialty
CHACMA BABOON
Platelet Function Tests
COAGULATION
Inflammation
03 medical and health sciences
Species Specificity
INFLAMMATION
biology.animal
Internal medicine
Thrombin receptor
Collagen/metabolism
medicine
Animals
Humans
PAR-1/metabolism
Receptor
PAR-1
HEMOSTASIS
Platelet Function Tests/methods
XENOTRANSPLANTATION
Receptor
PAR-1/metabolism
Thrombin/metabolism
AGGREGATION
ROTEM(R)
Peptide Fragments
Adenosine diphosphate
Endocrinology
chemistry
Platelet aggregometry
Hemostasis
Immunology
Receptors
Thrombin
Indicators and Reagents
Baboon
Papio
Zdroj: Thrombosis Research, 147, 115-121. Elsevier Science
ISSN: 0049-3848
Popis: BACKGROUND: Platelets play a pivotal role in coagulation, inflammation and wound healing. Suitable animal models that have the potential to mimic human platelet function are limited. The objective of the current study was to compare platelet aggregation response in the whole blood of baboons and humans using impedance aggregometry.METHODS: Blood was drawn from 24 anesthetised male baboons and 25 healthy volunteers. The platelet aggregation response was determined by impedance aggregometry (Multiplate®). Platelets in the hirudinised whole blood samples were stimulated with four different activators: adenosine diphosphate (ADP), collagen (COL), thrombin receptor activating peptide-6 (TR1AP), and activation of PAR-4 thrombin receptor subtype (TR4AP) at standard concentrations. Higher than standard concentrations were tested in a subgroup of the animals.RESULTS: The cell counts showed no differences between baboons and humans. The platelet aggregation response was significantly lower in baboons compared to humans when stimulated with the platelet agonists ADP (pCONCLUSION: The current study revealed that testing the platelet function in baboon blood by impedance aggregometry is feasible with ADP, COL and TR4AP, but not with TR1AP. Compared to humans, the aggregation response is lower in baboons. Considering the limitations in accordance to these results, baboons might represent a potential species for further platelet research.
Databáze: OpenAIRE
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