A simple approach for fabrication of optical affinity-based bioanalytical microsystem on polymeric PEN foils
| Autor: | Zdeňka Kolská, Petr Slepička, Ondřej Staněk, Jan Malý, Marcel Štofik, O. Neděla, Alena Semerádtová |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Streptavidin Materials science Optical Phenomena Surface Properties Serum albumin Biotin Nanotechnology 02 engineering and technology Naphthalenes 03 medical and health sciences chemistry.chemical_compound Colloid and Surface Chemistry medicine Humans Physical and Theoretical Chemistry Bovine serum albumin Polyethylene naphthalate Serum Albumin biology Photoelectron Spectroscopy Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology Human serum albumin 030104 developmental biology chemistry biology.protein Surface modification Microtechnology Protein G Polyethylenes 0210 nano-technology Biosensor Biotechnology medicine.drug |
| Zdroj: | Colloids and surfaces. B, Biointerfaces. 165 |
| ISSN: | 1873-4367 |
| Popis: | Herein, we report a novel concept of low-cost flexible platform for fluorescence-based biosensor. The surface of polyethylene naphthalate (PEN) foil was exposed to KrF excimer laser through a photolitographic contact mask. Laser initiated surface modification resulted in micro-patterned areas with surface functional groups available for localized covalent immobilization of biotin. High affinity binding protein (albumin-binding domain (ABD) of protein G, Streptococcus G148) recognizing human serum albumin (HSA), genetically fused with streptavidin (SA-ABDwt), was immobilized on the micro-patterned surface through biotin-streptavidin coupling. Fluorescently labelled HSA analyte was detected in several blocking environments, in 1% bovine serum albumin (BSA) and 6% fetal serum albumin (FBS), respectively. We conclude that the presented novel concept enabled us to micropattern functional biosensing layers on the surface of PEN foil in a fast and easy way. It brings all necessary aspects for continuous roll-to-roll fabrication of low-cost optical bioanalytical devices. |
| Databáze: | OpenAIRE |
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