Increased binding at 5-HT1A, 5-HT1B, and 5-HT2A receptors and 5-HT transporters in diet-induced obese rats
| Autor: | So-Young Park, Gareth Williams, Peter S. Widdowson, Joanne A. Harrold |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Ketanserin Tryptophan Hydroxylase Biology Midbrain Raphe Nuclei Radioligand Assay Internal medicine medicine Animals Receptor Serotonin 5-HT2A Obesity Rats Wistar Receptor Molecular Biology 5-HT receptor Neurons General Neuroscience Tryptophan hydroxylase Diet Rats Endocrinology Receptors Serotonin Receptor Serotonin 5-HT1B Raphe Nuclei Neurology (clinical) Serotonin Raphe nuclei Receptors Serotonin 5-HT1 Diet-induced obese Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 847:90-97 |
| ISSN: | 0006-8993 |
| Popis: | 5-Hydroxytryptamine (5-HT, serotonin), synthesized in midbrain raphe nuclei and released in various hypothalamic sites, decreases food intake but the specific 5-HT receptor subtypes involved are controversial. Here, we have studied changes in the regional density of binding to 5-HT receptors and transporters and the levels of tryptophan hydroxylase, in rats with obesity induced by feeding a palatable high-energy diet for 7 weeks. We mapped binding at 5-HT receptor subtypes and transporters using quantitative autoradiography and determined tryptophan hydroxylase protein levels by Western blotting. In diet-induced obese (DiO) rats, specific binding to 5-HT(1A) receptors ([3H]8-OH-DPAT) was significantly increased in the dorsal and median raphe by 90% (P |
| Databáze: | OpenAIRE |
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