Identification and antimicrobial suceptibility profile of bacteria causing bovine mastitis from dairy farms in Pelotas, Rio Grande do Sul

Autor: H. L. Gonzales, C. H. Freitas, P. V. Villarreal, Pedro Rassier dos Santos, Josiara Furtado Mendes, Carolina Lambrecht Gonçalves, Patrícia da Silva Nascente
Rok vydání: 2018
Předmět:
0301 basic medicine
Veterinary medicine
040301 veterinary sciences
Tetracycline
medicine.drug_class
Staphylococcus
030106 microbiology
Antibiotics
Drug resistance
Biology
0403 veterinary science
03 medical and health sciences
Antibiotic resistance
Anti-Infective Agents
lcsh:Botany
mastite bovina
Ampicillin
lcsh:Zoology
Drug Resistance
Bacterial
medicine
Animals
lcsh:QL1-991
Animal Husbandry
lcsh:Science
lcsh:QH301-705.5
Mastitis
Bovine
identification of microorganisms
04 agricultural and veterinary sciences
Staphylococcal Infections
bacterial resistance
Antimicrobial
medicine.disease
lcsh:QK1-989
Mastitis
Penicillin
Dairying
Milk
lcsh:Biology (General)
lcsh:Q
Cattle
Female
identificação de micro-organismos
General Agricultural and Biological Sciences
bovine mastitis
resistência bacteriana
medicine.drug
Zdroj: Brazilian Journal of Biology, Volume: 78, Issue: 4, Pages: 661-666, Published: 08 JAN 2018
Brazilian Journal of Biology v.78 n.4 2018
Brazilian Journal of Biology
Instituto Internacional de Ecologia (IIE)
instacron:IIE
Brazilian Journal of Biology, Issue: ahead, Published: 08 JAN 2018
Brazilian Journal of Biology, Iss 0 (2018)
ISSN: 1678-4375
1519-6984
DOI: 10.1590/1519-6984.170727
Popis: Mastitis is an inflammatory process of the udder tissue caused mainly by the bacteria Staphylococcus aureus. The indiscriminate use of antibiotics fosters conditions that favor the selection of resistant microorganisms, suppressing at the same time susceptible forms, causing a serious problem in dairy cattle. Given the importance in performing an antibiogram to select the most adequate antimicrobial therapy, the aim of this study was to identify bacteria isolated from cow’s milk with mastitis, in dairy farms situated in the city of Pelotas, Rio Grande do Sul, and to determinate the susceptibility profile of these isolates against the antibiotics used to treat this illness. A total of 30 isolates of Staphylococcus spp., were selected from milk samples from the udder quarters with subclinical mastitis whose species were identified through the Vitek system. The susceptibility profile was performed by the disk diffusion assay, against: ampicillin, amoxicillin, bacitracin, cephalexin, ceftiofur, enrofloxacin, gentamicin, neomycin, norfloxacin, penicillin G, tetracycline and trimethoprim. In the antibiogram, 100.0% of the isolates were resistant to trimethoprim and 96.7% to tetracycline and neomycin, three strains of Staphylococcus spp., (10.0%) presented resistance to the 12 antibiotics tested and 24 (80.0%) to at least eight. These results showed the difficulty in treating mastitis, due to the pathogens’ resistance. Resumo A mastite se constitui no processo inflamatório da glândula mamária causada principalmente por bactérias Staphylococcus aureus. O uso indiscriminado dos antibióticos promove condições que favorecem a seleção de micro-organismos resistentes e, ao mesmo tempo, suprime formas suscetíveis, causando um grave problema para a bovinocultura leiteira. Tendo em vista a importância da realização do antibiograma para a seleção da terapia antimicrobiana mais adequada, o objetivo deste estudo foi identificar bactérias isoladas de leite de vaca com mastite, oriundas de propriedades leiteiras localizadas na cidade de Pelotas, RS, bem como determinar o perfil de suscetibilidade desses isolados frente a antibióticos usados para o tratamento desta doença. Foram selecionados 30 isolados de Staphylococcus spp. de amostras de leite provenientes de quartos mamários com mastite subclínica, cujas espécies foram identificadas através do sistema Vitek. O perfil de suscetibilidade foi realizado pela técnica de difusão em disco, frente a: ampicilina, amoxicilina, bacitracina, cefalexina, ceftiofur, enrofloxacina, gentamicina, neomicina, norfloxacina, penicilina G, tetraciclina e trimetoprima. No antibiograma, 100,0% dos isolados foram resistentes a trimetoprima e 96,7% a tetraciclina e a neomicina, três cepas (10,0%) foram resistentes aos 12 antibióticos testados e 24 (80,0%) a pelo menos oito. Esses resultados demonstram a dificuldade encontrada no tratamento da mastite devido à resistência dos agentes patológicos.
Databáze: OpenAIRE
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