Quantum dot-based array for sensitive detection of Escherichia coli
| Autor: | Javier Adrian, Alfredo Sanz-Medel, Francisco Sánchez-Baeza, Nuria Pascual, Nuria Sanvicens, María Teresa Fernández-Argüelles, M.-Pilar Marco, José M. Costa-Fernández |
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| Rok vydání: | 2011 |
| Předmět: |
Detection limit
Chromatography biology Chemistry Analytical chemistry Escherichia coli O157 Microarray Analysis biology.organism_classification medicine.disease_cause Sensitivity and Specificity Biochemistry Fluorescence Enterobacteriaceae Orders of magnitude (mass) Analytical Chemistry Quantum dot Quantum Dots medicine Escherichia coli Bacteria Gram |
| Zdroj: | Analytical and Bioanalytical Chemistry. 399:2755-2762 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-010-4624-5 |
| Popis: | A fluorescent quantum dot-based antibody array, used in sandwich format, has been developed to detect Escherichia coli O157:H7. Numerous parameters such as solid support, optimal concentration of immunoreagents, blocking reagents, and assay time were optimized for array construction. Quantum dot-conjugated anti-IgG was used as the detecting system. The array allows the detection of E. coli O157:H7 at concentrations below 10 CFU mL(-1) without sample enrichment, exhibiting an increase of three orders of magnitude in the limit of detection compared to ELISA. The interference caused by Gram (+) and Gram (-) bacteria was negligible at low concentrations of bacteria. |
| Databáze: | OpenAIRE |
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