Transforming growth factor-?2polymorphisms are associated with childhood atopic asthma
| ISSN: | 1365-2222 0954-7894 |
|---|---|
| DOI: | 10.1111/j.1365-2222.2007.02768.x |
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5c85e66ea83f8d5483cd9e9d80f53e0 https://doi.org/10.1111/j.1365-2222.2007.02768.x |
| Rights: | CLOSED |
| Přírůstkové číslo: | edsair.doi.dedup.....e5c85e66ea83f8d5483cd9e9d80f53e0 |
| Autor: | Kenji Matsumoto, Keisuke Masuyama, Kimie Fujita, M. Ebisawa, Hiroshi Saito, Masafumi Sakashita, M. Harada, Tomomitsu Hirota, Ryohei Katoh, Mirei Kanzaki, Mayumi Tamari, Tadao Enomoto, Shinichi Takano, Takeshi Fukuda, Shigemi Yoshihara, Hideoki Ogawa, Satoru Doi, H. Sagara, Kyousuke Hatsushika, Atsuhito Nakao |
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male medicine.medical_specialty Linkage disequilibrium Allergy Adolescent Immunology Linkage Disequilibrium Cell Line Atopy Transforming Growth Factor beta2 Asian People Japan Internal medicine Immunopathology Genotype medicine Humans Immunology and Allergy Genetic Predisposition to Disease Child Promoter Regions Genetic 3' Untranslated Regions Aged Genetic association Asthma Polymorphism Genetic business.industry Middle Aged medicine.disease respiratory tract diseases Endocrinology Gene Expression Regulation Child Preschool Female business Transforming growth factor |
| Zdroj: | Clinical & Experimental Allergy. 37:1165-1174 |
| ISSN: | 1365-2222 0954-7894 |
| DOI: | 10.1111/j.1365-2222.2007.02768.x |
| Popis: | BACKGROUND: Transforming growth factor (TGF)-beta plays an important role in the regulation of airway inflammation and remodelling in asthma. Recent studies suggest that TGF-beta(2) is a predominant isoform expressed in severe asthma and it is also associated with airway remodelling. OBJECTIVE: To determine whether the polymorphisms in TGF-beta(2) are associated with childhood atopic bronchial asthma in a Japanese population. METHODS: We identified a total of eight polymorphisms and characterized the linkage disequilibrium (LD) mapping of the gene. Three variants in the promoter and 3'UTR were genotyped, and we conducted an association study of TGF-beta(2) (childhood atopic asthma n=297, normal controls n=555). An association analysis of these variants and an expression and functional analysis were performed. RESULTS: 3'UTR 94862T >A was found to be significantly associated with the risk of childhood atopic asthma (P=0.00041). The -109-->ACAA ins promoter variant was also associated with the risk of childhood atopic asthma (P=0.0037). TGF-beta(2) expression was observed in both the normal and asthmatic bronchial epithelium, and both real-time PCR and an ELISA showed a significant basal and TGF-beta(1)-induced TGF-beta(2) expression in the bronchial epithelial cell line BEAS2B. Furthermore, the promoter variant -109-->ACAA ins increased the TGF-beta(2) promoter-reporter activity in BEAS2B cells. CONCLUSIONS: Our data suggest that TGF-beta(2) may therefore be involved in the development of childhood atopic asthma by means of functional genetic polymorphism. The polymorphisms in TGF-beta(2) may become important information for asthma susceptibility in children. |
| Databáze: | OpenAIRE |
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