Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease
| Autor: | Xuejuan Gao, Hong-Ling Jia, Qi-Fang Song, Xiaoqiong Gu, Li Zhang, Ping Huang, Jing-Jie Jin, Zhou-Ping Wang, Xiao-Fei Xie |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Proteomics 0301 basic medicine China Apolipoprotein B Mucocutaneous Lymph Node Syndrome Exosomes Exosome Mass Spectrometry 03 medical and health sciences 0302 clinical medicine Intravenous Immunoglobulin Therapy medicine Humans Electrophoresis Gel Two-Dimensional Apolipoproteins A Innate immune system biology business.industry Immunoglobulins Intravenous Infant Blood Proteins Complement C3 General Medicine Hospitals Pediatric medicine.disease Complement system 030104 developmental biology Case-Control Studies Child Preschool 030220 oncology & carcinogenesis Pediatrics Perinatology and Child Health Immunology biology.protein Female Kawasaki disease Antibody Cardiology and Cardiovascular Medicine business Antibacterial humoral response Biomarkers |
| Zdroj: | Cardiology in the Young. 27:1786-1796 |
| ISSN: | 1467-1107 1047-9511 |
| Popis: | BackgroundKawasaki disease, which is characterised by systemic vasculitides accompanied by acute fever, is regularly treated by intravenous immunoglobulin to avoid lesion formation in the coronary artery; however, the mechanism of intravenous immunoglobulin therapy is unclear. Hence, we aimed to analyse the global expression profile of serum exosomal proteins before and after administering intravenous immunoglobulin.MethodsTwo-dimensional electrophoresis coupled with mass spectrometry analysis was used to identify the differentially expressed proteome of serum exosomes in patients with Kawasaki disease before and after intravenous immunoglobulin therapy.ResultsOur analysis revealed 69 differential protein spots in the Kawasaki disease group with changes larger than 1.5-fold and 59 differential ones in patients after intravenous immunoglobulin therapy compared with the control group. Gene ontology analysis revealed that the acute-phase response disappeared, the functions of the complement system and innate immune response were enhanced, and the antibacterial humoral response pathway of corticosteroids and cardioprotection emerged after administration of intravenous immunoglobulin. Further, we showed that complement C3 and apolipoprotein A-IV levels increased before and decreased after intravenous immunoglobulin therapy and that the insulin-like growth factor-binding protein complex acid labile subunit displayed reverse alteration before and after intravenous immunoglobulin therapy. These observations might be potential indicators of intravenous immunoglobulin function.ConclusionsOur results show the differential proteomic profile of serum exosomes of patients with Kawasaki disease before and after intravenous immunoglobulin therapy, such as complement C3, apolipoprotein A-IV, and insulin-like growth factor-binding protein complex acid labile subunit. These results may be useful in the identification of markers for monitoring intravenous immunoglobulin therapy in patients with Kawasaki disease. |
| Databáze: | OpenAIRE |
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