Clinicoepidemiologic pattern of cutaneous leishmaniasis and molecular characterization of its causative agent in Hajjah governorate, northwest of Yemen
| Autor: | Shabaan S. El Hossary, Bahira M. El Sawaf, Mishri Lal Khatri, Hala A. Kassem, Nadia F. Ramadan, Abdualdaim M. Mukred, Nabil M. Mogalli |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Yemen Leishmania tropica Adolescent Veterinary (miscellaneous) 030231 tropical medicine Leishmaniasis Cutaneous Environment Biology Moderate altitude Polymerase Chain Reaction Parasite Load Young Adult 03 medical and health sciences 0302 clinical medicine Cutaneous leishmaniasis Risk Factors parasitic diseases Epidemiology medicine Humans Leishmania species identification In patient 030212 general & internal medicine Child Aged Microscopy Case detection Transmission (medicine) Altitude Middle Aged medicine.disease biology.organism_classification Infectious Diseases Child Preschool Insect Science Female Parasitology Polymorphism Restriction Fragment Length |
| Zdroj: | Acta Tropica. 163:130-134 |
| ISSN: | 0001-706X |
| DOI: | 10.1016/j.actatropica.2016.08.012 |
| Popis: | The clinicoepidemiologic profile of 143 cases (93 males and 50 females) with cutaneous leishmaniasis from 18 villages of Hajjah governorate, Yemen was studied. Dry-type lesions were seen in 98.6% and wet-type lesions in 1.4% of patients. Lesions were localized in all cases with different morphological patterns. Microscopic examination of Giemsa-stained slit smears revealed amastigotes in 74.1% of patients with dry-type lesions and 0% in patients with wet-type lesions. The burden of the parasites in the lesions was high indicating active transmission of the disease. Most cases were from villages with moderate altitude range (8001-1600m). All age groups were affected, but most cases were seen in ages from 5 to 15 years. Leishmania species identification was done for all cases by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The biopsic material was scraped from both Giemsa-stained and methanol-fixed smears. The molecular characterization of Leishmania species revealed Leishmania tropica as the causative agent of cutaneous leishmaniasis in Hajjah, Yemen. The risk factors associated with the transmission of the disease and recommendations for improving case detection were discussed. |
| Databáze: | OpenAIRE |
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