Specificity of the extender used for freezing ram sperm depends of the spermatozoa source (ejaculate, electroejaculate or epididymis)
| Autor: | Luis Anel, S. Gomes-Alves, Felipe Martínez-Pastor, J. Tamayo-Canul, Mercedes Alvarez, C. Martínez-Rodríguez, E. López-Urueña, P. de Paz |
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| Přispěvatelé: | Biologia Celular, Facultad de Ciencias Biologicas y Ambientales |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Glycerol food.ingredient media_common.quotation_subject Fertility Biology Electroejaculation Cryopreservation law.invention Electroejaculate Andrology chemistry.chemical_compound Cryoprotective Agents Endocrinology food Food Animals Semen law Yolk Freezing medicine Animals Ejaculation Ejaculate media_common Epididymis Sheep Extender General Medicine Sperm Spermatozoa Electric Stimulation Ram medicine.anatomical_structure chemistry Animal Science and Zoology Veterinaria Egg yolk Semen Preservation |
| Popis: | P. 145-154 The objective of this study was to identify possible specificity in the extender formulation for the cryopreservation of ram spermatozoa recovered from three origins (ejaculate, electroejaculate or epididymis), by evaluating post-thawing sperm quality and fertility. Ejaculated, electroejaculated or epididymal spermatozoa samples obtained from identical rams (8) were cryopreserved in four different extenders (TES-Tris–fructose with one of two egg yolk concentrations: 10% Y10 and 20% Y20, and with one of two glycerol rates: 4% G4 and 8% G8). Samples were analyzed before and after cryopreservation by CASA (motility) and flow cytometry (viability with SYBR-14/PI and acrosomal status with PNA/PI). Spermatozoa obtained by electroejaculation were of poorer quality after freezing/thawing, demonstrating that protocols for these samples need to be optimized. Egg yolk at 20% was more appropriate for freezing sperm from any of the sources. In general, 4% glycerol improved the quality of post-thawing samples recovered from ejaculate and electroejaculate, while 8% glycerol was more appropriate for samples recovered from the epididymis. Based on these results, an analysis of fertility was conducted. Fertility rates were similar between ewe groups inseminated with post-thawed sperm obtained from two sources: ejaculate (cryopreserved in Y20 + G4), and cauda epididymis (Y20 + G8), and this rate was less in the electroejaculated sample (Y20 + G4) SI |
| Databáze: | OpenAIRE |
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