Characterization of zebrafish GABAA receptor subunits
| Autor: | Leona Shigemitsu, Kenichiro Sadamitsu, Marina Suzuki, Makoto Kashima, Hiromi Hirata, Daishi Ito |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Science Xenopus Protein subunit Genetic Vectors Gene Expression In situ hybridization Article Membrane Potentials Conserved sequence 03 medical and health sciences 0302 clinical medicine Animals Synaptic transmission Cloning Molecular Receptor Zebrafish Conserved Sequence In Situ Hybridization Fluorescence Phylogeny gamma-Aminobutyric Acid Multidisciplinary biology Chemistry GABAA receptor Zebrafish Proteins Receptors GABA-A biology.organism_classification Recombinant Proteins Cell biology Kinetics Protein Subunits 030104 developmental biology nervous system Larva Neuronal physiology Oocytes Chloride channel Medicine 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-84646-3 |
| Popis: | γ-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system, exerts its effect through the activation of GABA receptors. GABAA receptors are ligand-gated chloride channels composed of five subunit proteins. Mammals have 19 different GABAA receptor subunits (α1–6, β1–3, γ1–3, δ, ε, π, θ, and ρ1–3), the physiological properties of which have been assayed by electrophysiology. However, the evolutionary conservation of the physiological characteristics of diverged GABAA receptor subunits remains unclear. Zebrafish have 23 subunits (α1, α2a, α2b, α3–5, α6a, α6b, β1–4, γ1–3, δ, π, ζ, ρ1, ρ2a, ρ2b, ρ3a, and ρ3b), but the electrophysiological properties of these subunits have not been explored. In this study, we cloned the coding sequences for zebrafish GABAA receptor subunits and investigated their expression patterns in larval zebrafish by whole-mount in situ hybridization. We also performed electrophysiological recordings of GABA-evoked currents from Xenopus oocytes injected with one or multiple zebrafish GABAA receptor subunit cRNAs and calculated the half-maximal effective concentrations (EC50s) for each. Our results revealed the spatial expressions and electrophysiological GABA sensitivities of zebrafish GABAA receptors, suggesting that the properties of GABAA receptor subunits are conserved among vertebrates. |
| Databáze: | OpenAIRE |
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