Transcriptome-wide analysis of wild Asari (=Manila) clams affected by the Brown Muscle Disease: Etiology and impacts of the disease

Autor: X. de Montaudouin, Cindy Binias, Emilie Mérour, Michel Brémont, Patrice Gonzalez, Fabien Pierron, Anthony Bertucci
Přispěvatelé: Faculty of Engineering and the Environment (µVIS X-ray Imaging Centre), University of Southampton, Unité de virologie et immunologie moléculaires, Institut National de la Recherche Agronomique (INRA), UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Nervous system
animal structures
Venerupis phillippinarum
Physiology
Disease
Aquatic Science
Biology
Synaptic Transmission
Transcriptome
03 medical and health sciences
brown muscle disease
Atrophy
Muscular Diseases
rna-Seq
Ruditapes phillippinarurn
neurotoxicity
Neurotoxicity
medicine
Environmental Chemistry
Animals
neurotoxicity. 1. Introduction
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
RNA-Seq
14. Life underwater
Gene
ComputingMilieux_MISCELLANEOUS
Sequence Analysis
RNA
04 agricultural and veterinary sciences
General Medicine
medicine.disease
Muscle atrophy
Bivalvia
030104 developmental biology
medicine.anatomical_structure
Retroviridae
Brown Muscle Disease
040102 fisheries
Etiology
0401 agriculture
forestry
and fisheries
France
Adductor muscles
medicine.symptom
ruditapes phillippinarurn
Retroviridae Infections
Zdroj: Fish and Shellfish Immunology
Fish and Shellfish Immunology, Elsevier, 2019, 86, pp.179-185. ⟨10.1016/j.fsi.2018.11.043⟩
Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd-Elsevier Science Ltd), 2019-03, Vol. 86, P. 179-185
ISSN: 1050-4648
1095-9947
Popis: International audience; Recently, we reported an emerging pathology named Brown Muscle Disease (BMD) affecting Asari clams inhabiting the most productive area for this species in France, the Arcachon Bay. The main macroscopic feature of the pathology relies on the atrophy of the posterior adductor muscle, affecting the ability of clams to burry. The research of the etiological agent of BMD privileged a viral infection. Contrary to healthy clams, infected animals are always found at the surface of the sediment and exhibit 30 nm virus-like particles in muscle, granulocytic and rectal cells. In order to get more insights on the etiology and impacts of the BMD on clams, we took advantage in the present study of next generation sequencing technologies. An RNA-Seq approach was used (i) to test whether viral RNA sequences can be specifically found in the transcriptome of diseased animals and (ii) to identify the genes that are differentially regulated between diseased and healthy clams. Contrary to healthy buried animals, in diseased clams one sequence showing extensive homologies with retroviridae-related genes was detected. Among the biological processes that were affected in diseased clams, the synaptic transmission process was the most represented. To deepen this result, a new sampling was carried out and the transcription level of genes involved in synaptic transmission was determined in healthy and diseased clams but also in clams with no visible sign of pathology but located at the surface of the sediment. Our findings suggest that muscle atrophy is a latter sign of the pathology and that nervous system could be instead a primary target of the BMD agent.
Databáze: OpenAIRE
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