Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor
| Autor: | Cemal Ün, Bayram Pektaş, Mert Döşkaya, Aysu Değirmenci Döşkaya, Tülay Öncü Öner, Hüseyin Can, Selçuk Kaya, Muhammet Karakavuk, Mehmet Karabey, Adnan Yüksel Gürüz, Ahmet Alacacıoğlu, Ayşegül Aksoy Gökmen, Aytül Gül, Ahmet Efe Köseoğlu, Sedef Erkunt Alak |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Diarrhea
Cross-sectional study Specimens medicine.medical_treatment Vertebrate Animals Sensitivity parasitic diseases medicine Prevalence Parasites Solid tumor Cancer Chemotherapy Microscopy biology business.industry Pneumocystis Cryptosporidium General Medicine biology.organism_classification Protozoan parasite Pcr Immunology Medicine Original Article medicine.symptom business Infection |
| Zdroj: | Annals of Saudi Medicine, Vol 41, Iss 5, Pp 293-298 (2021) Annals of Saudi Medicine |
| Popis: | BACKGROUND: Cryptosporidium spp. is a protozoan parasite that infects many vertebrate animals, including humans. Since Cryptosporidium spp. can cause chronic life-threatening diarrhea and severe malabsorption in immunocompromised patients, we investigated the prevalence of this parasite among patients undergoing chemotherapy for malignant solid tumors. OBJECTIVE: Investigate the prevalence of Cryptosporidium spp. in stool samples. DESIGN: Cross-sectional. SETTING: Tertiary care. PATIENTS AND METHODS: Stool samples were collected from adult patients with malignant solid tumors receiving chemotherapy and diarrhea. Cryptosporidium spp. prevalence was determined using Ziehl-Neelsen staining, ELISA, and real-time PCR targeting of the COWP gene. MAIN OUTCOME MEASURE: The prevalence of Cryptosporidium spp. in patients undergoing chemotherapy for malignant solid tumors. SAMPLE SIZE: 94 RESULTS: The prevalence was 2.1% (2/94), 5.3% (5/94), and 5.3% (5/94) as detected by Ziehl-Neelsen staining, real-time PCR and ELISA, respectively. The prevalence reached 8.5% (8/94) using all results obtained from the three methods. Among eight positive stool samples, four were positive by at least two different methods (Ziehl-Neelsen staining-ELISA or ELISA-real-time PCR) whereas the remaining four were positive by either ELISA or real-time PCR. CONCLUSION: These findings show the risk of cryptosporidiosis in cancer patients and the necessity to use at least two diagnostic methods during the diagnosis of cryptosporidiosis to reach more accurate and trustworthy results. LIMITATIONS: Further studies with a larger sample size are recommended. CONFLICT OF INTEREST: None. Izmir Katip Celebi University Scientific Research Projects Coordination Unit [x 2018-TDU-TIPF-0055] Izmir Katip Celebi University Scientific Research Projects Coordination Unit x 2018-TDU-TIPF-0055 to A.A. |
| Databáze: | OpenAIRE |
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