MiR-122 is involved in immune response by regulating Interleukin-15 in the orange-spotted grouper (Epinephelus coioides)
| Autor: | Nina Wei, QingJian Liang, Xing Liu, Yan Hao, YinHua Liu, LiPing Peng |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fish Proteins
0301 basic medicine Orange-spotted grouper Spleen Aquatic Science Biology Microbiology Fish Diseases 03 medical and health sciences Immune system medicine Animals Environmental Chemistry Grouper Phylogeny Interleukin-15 Base Sequence Cell growth Gene Expression Profiling 04 agricultural and veterinary sciences General Medicine Epinephelus biology.organism_classification Immunity Innate Aeromonas hydrophila MicroRNAs 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Interleukin 15 040102 fisheries 0401 agriculture forestry and fisheries Bass Gram-Negative Bacterial Infections Sequence Alignment |
| Zdroj: | Fish & Shellfish Immunology. 106:404-409 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2020.08.021 |
| Popis: | Epinephelus coioides is an important economic culture marine fish and is susceptible to various pathogenic diseases. Increasingly evidences showed that miRNAs participated in the regulation of the cell proliferation, differentiation and immune response. MiR-122 has been reported to play an essential role in immune response by triggering an inflammatory reaction. However, the function of miR-122 in response to bacterial infection is unclear in Epinephelus coioides. Herein, we report that miR-122 is involved in response to Aeromonas hydrophila infection of grouper spleen cells (GS). IL-15, IL-6 and IL-1β are inhibited in overexpression miR-122 GS cells, while induced in silence miR-122 GS cells. In addition, IL-15 is predicted to be the target gene of miR-122, which is further confirmed by LUC. Taken together, we propose that miR-122 regulates the immune response to bacterial infection by triggering IL-15. |
| Databáze: | OpenAIRE |
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