Pseudomonas Quinolone Signal Induces Oxidative Stress and Inhibits Heme Oxygenase-1 Expression in Lung Epithelial Cells
| Autor: | Melissa Bavitz, Maher Y. Abdalla, Barbara L. Switzer, Bradley E. Britigan, Traci Hoke, Peter J. Murphy, Javier Seravalli, Jill D. Fliege |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Immunology Quinolones Biology medicine.disease_cause Microbiology Virulence factor Cell Line Flow cytometry Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Annexin medicine Animals Humans Propidium iodide Enzyme Inhibitors chemistry.chemical_classification Reactive oxygen species Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Microscopy Confocal medicine.diagnostic_test Macrophages Epithelial Cells respiratory system Flow Cytometry Heme oxygenase Oxidative Stress 030104 developmental biology Infectious Diseases chemistry Apoptosis 030220 oncology & carcinogenesis Parasitology Reactive Oxygen Species Heme Oxygenase-1 Oxidative stress |
| Zdroj: | Infection and Immunity. 85 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00176-17 |
| Popis: | Pseudomonas aeruginosa causes lung infections in patients with cystic fibrosis (CF). The Pseudomonas quinolone signal (PQS) compound is a secreted P. aeruginosa virulence factor that contributes to the pathogenicity of P. aeruginosa . We were able to detect PQS in sputum samples from CF patients infected with P. aeruginosa but not in samples from uninfected patients. We then tested the hypothesis that PQS induces oxidative stress in host cells by determining the ability of PQS to induce the production of reactive oxygen species (ROS) in lung epithelial cells (A549 and primary normal human bronchial epithelial [NHBE]) cells and macrophages (J774A.1 and THP-1). ROS production induced by PQS was detected with fluorescent probes (dichlorodihydrofluorescein diacetate, dihydroethidium, and MitoSOX Red) in conjunction with confocal microscopy and flow cytometry. PQS induced ROS production in lung epithelial (A549 and NHBE) cells and macrophages (J774A.1 and THP-1 cells). NHBE cells were sensitive to PQS concentrations as low as 500 ng/ml. PQS significantly induced early apoptosis ( P < 0.05, n = 6) in lung epithelial cells, as measured by annexin/propidium iodide detection by flow cytometry. However, no change in apoptosis upon PQS treatment was seen in J774A.1 cells. Heme oxygenase-1 (HO-1) protein is an antioxidant enzyme usually induced by oxidative stress. Interestingly, incubation with PQS significantly reduced HO-1 and NrF2 expression in A549 and NHBE cells but increased HO-1 expression in J774A.1 cells ( P < 0.05, n = 3), as determined by immunoblotting and densitometry. These PQS effects on host cells could play an important role in the pathogenicity of P. aeruginosa infections. |
| Databáze: | OpenAIRE |
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