Double Transgenic Mice Crossed GFP-LC3 Transgenic Mice With .ALPHA.MyHC-mCherry-LC3 Transgenic Mice Are a New and Useful Tool to Examine the Role of Autophagy in the Heart
| Autor: | Yoshiko Munehisa, Yoichiro Takahashi, Hiroshi Ito, Kenji Iino, Takashi Koyama, Toshimitsu Kosaka, Mai Terada, Hiroyuki Watanabe, Yutaka Terata, Masaru Ishida, Kiyoshi Nobori, Takayoshi Ohba, Manabu Kakizaki, Hitoshi Hasegawa |
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| Rok vydání: | 2010 |
| Předmět: |
Genetically modified mouse
Autophagosome maturation Transgene Green Fluorescent Proteins Myocardial Ischemia Apoptosis Mice Transgenic Biology Green fluorescent protein Mice Autophagy Animals Myosin Heavy Chains Myocardium Myocardium metabolism General Medicine Molecular biology Disease Models Animal embryonic structures biological phenomena cell phenomena and immunity Cardiomyopathies Cardiology and Cardiovascular Medicine mCherry Microtubule-Associated Proteins |
| Zdroj: | Circulation Journal. 74:203-206 |
| ISSN: | 1347-4820 1346-9843 |
| DOI: | 10.1253/circj.cj-09-0589 |
| Popis: | Background: The involvement of autophagy in heart disease has been reported. Transgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice. Methods and Results: We generated transgenic mice expressing mCherry-LC3 under αMyHC promoter and crossed the mice with transgenic mice expressing GFP-LC3. The deference of resistance to acidic conditions between GFP and mCherry overcame the limitation. Conclusions: This method is an innovative approach to examine the role of autophagy and to analyze autophagosome maturation in cardiomyocytes. (Circ J 2010; 74: 203 - 206) |
| Databáze: | OpenAIRE |
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