Micronucleus frequency is correlated with antioxidant enzyme levels in workers occupationally exposed to pesticides
| Autor: | Monserrat Sordo, Irma Martha Medina-Díaz, Mirna Citlali Guerrero-Flores, José Francisco Herrera-Moreno, Aurora Elizabeth Rojas-García, Maria del Carmen Xotlanihua-Gervacio, B.S. Barrón-Vivanco, Y.Y. Bernal-Hernández |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Antioxidant Health Toxicology and Mutagenesis medicine.medical_treatment Glutathione reductase Air Pollutants Occupational medicine.disease_cause Antioxidants Superoxide dismutase 03 medical and health sciences Internal medicine Occupational Exposure medicine Environmental Chemistry Humans Pesticides chemistry.chemical_classification Glutathione Peroxidase Micronucleus Tests biology Superoxide Dismutase Glutathione peroxidase General Medicine Catalase Pollution Oxidative Stress 030104 developmental biology Endocrinology Cross-Sectional Studies Glutathione Reductase chemistry Micronucleus test biology.protein Female Micronucleus Oxidation-Reduction Oxidative stress DNA Damage |
| Zdroj: | Environmental science and pollution research international. 25(31) |
| ISSN: | 1614-7499 |
| Popis: | Oxidative stress can cause DNA damage leading to nuclear anomalies such as micronuclei (MN). Antioxidant enzymes involved in protection against intracellular oxidative stress include glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT). Pesticide exposure induces oxidative stress and alters antioxidant defense mechanisms, including detoxification and scavenger enzymes. The aim of this study was to evaluate MN frequency in workers occupationally exposed to pesticides and their relationship with antioxidant enzyme activities. A cross-sectional study was conducted in 201 individuals, some of whom were dedicated to the spraying of pesticides. The cytokinesis-block micronucleus (CBMN) assay was conducted, and the activities of GPx, GR, SOD, and CAT were determined. The geometric mean (GM) of MN was 5.4 (1–26 MN). The GM for the antioxidant enzymes was 198.68 U/mL for GPx, 38.96 U/g Hb for GR, 94.78 U/mL for SOD, and 69.77 U/g Hb for CAT. There was a lower MN frequency in males than that in females, and a higher nuclear index. In addition, age affected MN frequency. There was a negative correlation between MN frequency and GPx activity, but a positive one between MN frequency and GR activity. These findings suggest the involvement of GPx in MN frequency. |
| Databáze: | OpenAIRE |
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