Validity of leptin receptor-deficiency (db/db) type 2 diabetes mellitus mice as a model of secondary osteoporosis
| Autor: | Le Huang, Lizhen Zheng, Dong Yao, Hui-Yao Lan, Ling Qin, Yong-Ke You, Hai-Yong Chen, Xiao-Ru Huang, Tracy Y. Zhu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Bone density 030209 endocrinology & metabolism Article Bone and Bones 03 medical and health sciences Mice 0302 clinical medicine Imaging Three-Dimensional Bone Density Diabetes mellitus Internal medicine medicine Animals Bone mineral Mice Knockout Multidisciplinary Leptin Deficiency business.industry Leptin Body Weight Type 2 Diabetes Mellitus Reproducibility of Results X-Ray Microtomography medicine.disease Biomechanical Phenomena Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure Diabetes Mellitus Type 2 Cancellous Bone Osteoporosis Receptors Leptin Cortical bone Secondary osteoporosis business |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | This study aimed to evaluate the validation of the leptin receptor-deficient mice model for secondary osteoporosis associated with type 2 diabetes mellitus (T2DM) at bone micro-architectural level. Thirty three 36-week old male mice were divided into four groups: normal control (db/m) (n = 7), leptin receptor-deficient T2DM (db/db) (n = 8), human C-reactive protein (CRP) transgenic normal control (crp/db/m) (n = 7) and human CRP transgenic T2DM (crp/db/db) (n = 11). Lumber vertebrae (L5) and bilateral lower limbs were scanned by micro-CT to analyze trabecular and cortical bone quality. Right femora were used for three-point bending to analyze the mechanical properties. Trabecular bone quality at L5 was better in db/db or crp/db/db group in terms of bone mineral density (BMD), bone volume fraction, connectivity density, trabecular number and separation (all p crp/db/db group was significantly shorter than db/m group (p db/db and crp/db/db groups (p > 0.05). Maximum loading and energy yield in mechanical test were similar among groups while the elastic modulus in db/db and crp/db/db significantly lower than db/m. The leptin-receptor mice is not a proper model for secondary osteoporosis associated with T2DM. |
| Databáze: | OpenAIRE |
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