Clinostation influence on regeneration of cell wall in Solanum tuberosum L. protoplasts
| Autor: | V.A. Sidorov, V.M. Samoylov, E.M. Nedukha |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Atmospheric Science
Chloroplasts Rotation Aerospace Engineering Matrix (biology) Calcofluor-white Cell wall Cell Wall Regeneration Weightlessness Simulation Solanum tuberosum Organelles Chemistry Regeneration (biology) Protoplasts fungi food and beverages Astronomy and Astrophysics Protoplast Staining Microscopy Electron Geophysics Space and Planetary Science Biophysics General Earth and Planetary Sciences Clinostat Gravitation |
| Zdroj: | Advances in space research : the official journal of the Committee on Space Research (COSPAR). 14(8) |
| ISSN: | 0273-1177 |
| Popis: | Regeneration of cell walls in protoplasts was investigated using light- and electronmicroscopic methods. The protoplasts were isolated from mesophyll of Solanum tuberosum leaves and were cultivated on the horizontal low rotating clinostat (2 rpm) and in control for 10 days. Using a fluorescent method (with Calcofluor white) it was demonstrated that changes in vector gravity results in an regeneration inhibition of cell wall. With electron-microscopical and electro-cytochemical methods (staining with alcianum blue) dynamics of the regeneration of cell walls in protoplasts was studied; carbohydrate matrix of cell walls is deposited at the earliest stages of this process. The influence of microgravity on the cell wall regeneration is discussed in higher plants. |
| Databáze: | OpenAIRE |
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