Regenerative Adaptation to Electrochemical Perturbation in Planaria: A Molecular Analysis of Physiological Plasticity
| Autor: | Anna Kane, Christopher J. Martyniuk, Fallon Durant, Angela Tung, Maya Emmons-Bell, Junji Morokuma, Kelsie A. Miller, Michael Levin, Alexis Pietak, Devon Davidian |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell physiology Excitotoxicity 02 engineering and technology medicine.disease_cause Marine Organism Article 03 medical and health sciences TRPM medicine Ion Activity lcsh:Science Phenotypic plasticity Multidisciplinary biology Voltage-dependent calcium channel 021001 nanoscience & nanotechnology biology.organism_classification Planaria Cell biology 030104 developmental biology Planarian Dugesia japonica lcsh:Q Cellular Physiology 0210 nano-technology |
| Zdroj: | iScience, Vol 22, Iss, Pp 147-165 (2019) iScience |
| ISSN: | 2589-0042 |
| Popis: | Summary Anatomical homeostasis results from dynamic interactions between gene expression, physiology, and the external environment. Owing to its complexity, this cellular and organism-level phenotypic plasticity is still poorly understood. We establish planarian regeneration as a model for acquired tolerance to environments that alter endogenous physiology. Exposure to barium chloride (BaCl2) results in a rapid degeneration of anterior tissue in Dugesia japonica. Remarkably, continued exposure to fresh solution of BaCl2 results in regeneration of heads that are insensitive to BaCl2. RNA-seq revealed transcriptional changes in BaCl2-adapted heads that suggests a model of adaptation to excitotoxicity. Loss-of-function experiments confirmed several predictions: blockage of chloride and calcium channels allowed heads to survive initial BaCl2 exposure, inducing adaptation without prior exposure, whereas blockade of TRPM channels reversed adaptation. Such highly adaptive plasticity may represent an attractive target for biomedical strategies in a wide range of applications beyond its immediate relevance to excitotoxicity preconditioning. Graphical Abstract Highlights • Exposure to BaCl2 causes the heads of Dugesia japonica to degenerate • Prolonged exposure to BaCl2 results in regeneration of a BaCl2-insensitive head • Ion channel expression is altered in the head to compensate for excitotoxic stress • TRPMa is upregulated in BaCl2-treated animals; blocking TRPM prevents adaptation Marine Organism; Ion Activity; Cellular Physiology |
| Databáze: | OpenAIRE |
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