Recovery of nicotine-free proteins from tobacco leaves using phosphate buffer system under controlled conditions
| Autor: | Tae-Shik Hahm, Hong Fu, Robert J. Kratochvil, Y.M. Lo, Cheng-i Wei, Peter A. Machado |
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| Rok vydání: | 2010 |
| Předmět: |
Nicotine
Environmental Engineering Ultrafiltration Bioengineering Buffers Chemistry Techniques Analytical Phosphates Acetone chemistry.chemical_compound Tobacco medicine Phosphoric Acids Waste Management and Disposal Phosphoric acid Plant Proteins Chromatography Renewable Energy Sustainability and the Environment Alkaloid Temperature Agriculture General Medicine Hydrogen-Ion Concentration Contamination Phosphate Plant Leaves chemistry Yield (chemistry) Biotechnology medicine.drug |
| Zdroj: | Bioresource Technology. 101:2034-2042 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2009.10.045 |
| Popis: | Establishment of an effective, high-throughput processing system to recover protein from tobacco with no nicotine contamination is essential and vital to the development of value-added, alternative applications for tobacco farmers. We have successfully developed a mechanism capable of processing up to 60 kg of tobacco leaves per hour with phosphate buffer (Na(2)HPO(4)-KH(2)PO(4)) simultaneously added to stabilize the protein as the plant was being disintegrated. The optimal processing parameters were identified, including the ratio of buffer to leaf (BLR) at 4.75 (w/w), buffer pH 7.85, and buffer concentration 0.085 mol/L, achieving a maximum yield of soluble protein at 12.85 mg/g fresh leaf. Acetone at -20 degrees C was the most effective among all methods investigated to remove nicotine from protein; however, it also drastically reduced the recovery rate of protein (63.3%). Ultrafiltration was only able to remove about 50% of the residual nicotine, although the protein recovery rate was high (94.7%). The residual nicotine content inherent in the recovered protein was completely removed by rinsing the protein with 85% phosphoric acid at pH 3.5 for three times with a protein recovery of 94.5%. The pilot-scale operation provides a solid foundation for further scale-up to industrial production of nicotine-free tobacco protein that could bring added value to tobacco for nonsmoking applications. |
| Databáze: | OpenAIRE |
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