Characterization of three casein kinases type I from Dictyostelium discoideum
| Autor: | Margarita Fernández-Renart, Blanca Ospina |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
biology
GTP' Heparin Autophosphorylation Cell Biology biology.organism_classification Molecular biology Dictyostelium discoideum Kinetics Biochemistry Casein Casein kinase 2 alpha 1 Centrifugation Density Gradient Phosphorylation Dictyostelium Casein kinase 1 Amino Acids Casein kinases Casein Kinases Protein Kinases Molecular Biology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/0167-4889(90)90159-b |
| Popis: | Three different casein kinases type I have been characterized and partially purified from vegetative cells of Dictyostelium discoideum. The enzymes have been classified as type I because they are excluded from DEAE cellulose columns and do not utilize GTP as phosphoryl donor. We have named these activities as casein kinases IA, IB and IC respectively, according to the elution profile on phosphocellulose chromatography. The three activities differ in: the sensitivity to heparin inhibition; the salt optimum for activity and the amino acids phosphorylated, using casein as substrate. Experiments carried out in conditions that favor autophosphorylation indicate that casein kinase IB could have a 53 kDa subunit, susceptible to autphosphorylation in vitro. This investigation was supported by a CAICYT grant. B.O. is a fellow from the I.C.I. |
| Databáze: | OpenAIRE |
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