Detection of Human Papillomaviruses in the Nasopharynx of Breastfed Infants: New Findings and Meta-Analysis
| Autor: | Maria Lina Tornesello, Noemy Starita, Chiara Botti, Alberto Micillo, Andrea Cerasuolo, Luisa Dassi, Franco M. Buonaguro, Clorinda Annunziata |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Genotype breastfeeding viruses Breastfeeding lcsh:QR1-502 Physiology Alphapapillomavirus Breast milk Polymerase Chain Reaction Article lcsh:Microbiology 03 medical and health sciences 0302 clinical medicine Pregnancy alpha HPVs Nasopharynx Virology beta HPVs Prevalence Humans Medicine Respiratory Tract Infections Skin Transmission (medicine) Vaginal delivery business.industry infants Papillomavirus Infections Infant Newborn Infant virus diseases Infectious Disease Transmission Vertical female genital diseases and pregnancy complications human papillomavirus (HPV) Breast Feeding 030104 developmental biology Infectious Diseases 030220 oncology & carcinogenesis DNA Viral Female Sample collection Recurrent Respiratory Papillomatosis business Nested polymerase chain reaction |
| Zdroj: | Viruses, Vol 12, Iss 1119, p 1119 (2020) Viruses Volume 12 Issue 10 |
| ISSN: | 1999-4915 |
| Popis: | Vertical transmission of human papillomaviruses (HPVs) from mother to infant is known to occur during labor, delivery or breastfeeding. Infection with mucosal HPV 6 and 11 may cause recurrent respiratory papillomatosis in children, which is a rare and severe respiratory disease. The cutaneous HPV genotypes have also been described to be transmitted from mother to newborn through skin-to-skin contacts and during breastfeeding. To investigate the perinatal transmission of alpha and beta HPVs we collected nasopharyngeal specimens from 0&ndash 12-months-old infants born by vaginal delivery and breastfed at the time of sample collection. The mucosal and cutaneous HPVs were searched by nested PCR using the MY09/11-MGPs and CP65/70-CP66/69 primer sets, respectively, and genotypes identified by direct sequencing analysis. Fourteen out of 113 (12.4%) samples tested positive for HPV and sequence analysis allowed us to identify eight beta genotypes (HPV 5b, 20, 25, 100, 107, 124, 152 and RTRX7). Moreover, we performed a comprehensive review of published studies on the prevalence of mucosal and cutaneous HPVs among 5126 newborns and observed that 10% and 53% were positive for alpha and beta HPVs, respectively. In all studies there was an inverse correlation between the rate of alpha HPV positivity and age, while a significant positive trend was observed in beta HPV detection and age with the highest rate among children older than 12 months (&Chi 2 test for trend of 10.6, p < 0.001). Further studies are needed to confirm the hypothesis that beta HPVs are transmitted to breastfeeding infants through shedding of viruses in the breast milk or on the external breast epithelium. |
| Databáze: | OpenAIRE |
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