Development and Chimpanzee Testing of a Vaccine against Human Hepatitis B
| Autor: | Robert R. Roehm, Alexander U. Bertland, Maurice R. Hilleman, Lampson Gp, Eugene B. Buynak, Alfred A. Tytell |
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| Rok vydání: | 1976 |
| Předmět: |
Hepatitis B virus
Hepatitis B vaccine Pan troglodytes Guinea Pigs Antibodies Viral medicine.disease_cause General Biochemistry Genetics and Molecular Biology Hepatitis B Antigens Blood serum Antigen medicine Animals Hepatitis B Antibodies Hepatitis Vaccines biology Haplorhini Hepatitis B medicine.disease Virology Vaccination Immunology biology.protein Antibody |
| Zdroj: | Experimental Biology and Medicine. 151:694-700 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | Highly purified hepatitis B virus surface antigen (Australia antigen) purified by physical and chemical procedures from infected human plasma was used to prepare hepatitis B vaccine. The purified antigen was treated with formalin and the vaccine was tested exhaustively for safety by ordinary procedures and additionally in marmosets (for live hepatitis B virus). The vaccine was highly potent, inducing antibody in guinea pigs, grivet monkeys, and chimpanzees given three doses of vaccine containing up to 20 mug of hepatitis B antigen per dose. A protective efficacy trial was carried out in chimpanzees that were given three doses of vaccine subcutaneously and then challenged intravenously with 1000 chimpanzee infectious doses of human hepatitis B virus. All of five unvaccinated control animals developed hepatitis B virus antigenemia following challenge and all of six vaccinated animals were protected, including one animal that had failed to develop detectable antibody following vaccination. |
| Databáze: | OpenAIRE |
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