L-ascorbic acid 2-phosphate, a phosphate derivative of L-ascorbic acid, enhances the growth of cultured rabbit keratocytes
| Autor: | Shizuya Saika, K. Uenoyama, Jun-ichi Hiraoka, R. Kanagawa, Kenji Hiroi |
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| Rok vydání: | 1991 |
| Předmět: |
Vitamin
Keratinocytes Male endocrine system Stromal cell animal diseases Corneal Stroma Ascorbic Acid Biology Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Animals Proline Cells Cultured Vitamin C hemic and immune systems Phosphate Ascorbic acid eye diseases Sensory Systems Ophthalmology medicine.anatomical_structure chemistry Biochemistry Cell culture Female Rabbits Keratinocyte tissues Azetidinecarboxylic Acid Cell Division |
| Zdroj: | Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie. 229(1) |
| ISSN: | 0721-832X |
| Popis: | We examined the effect of L-ascorbic acid 2-phosphate (P-Asc) on the proliferation of cultured rabbit keratocytes. P-Asc is a phosphate derivative of L-ascorbic acid and has more prolonged vitamin C activity in solution than does L-ascorbic acid. The proliferation of cultured keratocytes was promoted by the presence of P-Asc in culture medium. Transmission electron microscopic observations revealed that cells were more multi-layered after culture in the presence of P-Asc (0.1 mM) for 30 days than were those cultured in the absence of P-Asc. The effect of P-Asc was abrogated by L-azetidine 2-carboxylic acid, which is an analogue of proline that inhibits the production and secretion of collagen. Our observations support a therapeutic role for P-Asc in the repair of corneal stromal damage such as that caused by a corneal chemical burn. |
| Databáze: | OpenAIRE |
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