Resveratrol Protects against 2-Bromopropane-Induced Apoptosis and Disruption of Embryonic Development in Blastocysts
| Autor: | Wen-Hsiung Chan |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Cell Survival
Embryonic Development resveratrol Biology Resveratrol Protective Agents Article Antioxidants Catalysis lcsh:Chemistry Inorganic Chemistry Mice chemistry.chemical_compound Pregnancy In vivo Stilbenes medicine Animals 2-Bromopropane Embryo Implantation Blastocyst Physical and Theoretical Chemistry development lcsh:QH301-705.5 Molecular Biology Spectroscopy Cell Proliferation chemistry.chemical_classification Reactive oxygen species Dose-Response Relationship Drug blastocyst Cell growth Organic Chemistry Embryogenesis apoptosis General Medicine In vitro Hydrocarbons Brominated Computer Science Applications Cell biology medicine.anatomical_structure lcsh:Biology (General) lcsh:QD1-999 chemistry Biochemistry Apoptosis Dietary Supplements Female Reactive Oxygen Species |
| Zdroj: | International Journal of Molecular Sciences, Vol 12, Iss 8, Pp 4991-5010 (2011) International Journal of Molecular Sciences Volume 12 Issue 8 Pages 4991-5010 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms12084991 |
| Popis: | 2-Bromopropane (2-BP) is used as an alternative to ozone-depleting cleaning solvents. Previously, we reported that 2-BP has cytotoxic effects on mouse blastocysts and is associated with defects in subsequent development. In the present work, we show that 2-BP induces apoptosis in the inner cell mass of mouse blastocysts, and inhibits cell proliferation. Both effects are suppressed by resveratrol, a grape-derived phytoalexin with known antioxidant and anti-inflammatory properties. 2-BP-treated blastocysts displayed lower levels of implantation (compared to controls) when plated on culture dishes in vitro, and a reduced ability to proceed to later stages of embryonic development. Pretreatment with resveratrol prevented 2-BP-induced disruption of embryonic development, both in vitro and in vivo. Further investigation of these processes revealed that 2-BP directly promotes ROS generation, loss of mitochondrial membrane potential (MMP), and activation of caspase-3, whereas resveratrol effectively blocks 2-BP-induced ROS production and the accompanying apoptotic biochemical changes. Our results collectively imply that 2-BP triggers the mitochondrion-dependent apoptotic pathway via ROS generation, and the antioxidant activity of resveratrol prevents 2-BP-induced toxicity. |
| Databáze: | OpenAIRE |
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