Silencing of ZRF1 impedes survival of estrogen receptor positive MCF-7 cells and potentiates the effect of curcumin
| Autor: | Vijayalaxmi Kari, Dipta Sengupta, Swayamsiddha Kar, Nibedita Pradhan, Sabnam Parbin, Sandip Kumar Rath, Moonmoon Deb, Samir Kumar Patra |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Jumonji Domain-Containing Histone Demethylases Curcumin Estrogen receptor Apoptosis Breast Neoplasms Biology Bioinformatics 03 medical and health sciences Breast cancer Downregulation and upregulation Cell Movement medicine Humans Viability assay Protein kinase B Cell Proliferation Oncogene Proteins Gene knockdown Cell growth RNA-Binding Proteins General Medicine medicine.disease DNA-Binding Proteins 030104 developmental biology MCF-7 Proto-Oncogene Proteins c-bcl-2 Receptors Estrogen Cancer research MCF-7 Cells Female Proto-Oncogene Proteins c-akt Molecular Chaperones |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 37(9) |
| ISSN: | 1423-0380 |
| Popis: | The role and clinical implication of ZRF1 in breast cancer are poorly understood. So this study is aimed to explore the role of ZRF1 in breast cancer progression. With this context, we first assessed its expression pattern in FFPE primary and metastasis breast tissue samples as well as from publicly available databases. Moreover, we also explored the survival status of patients from the publicly available database and interestingly discover that high expression of ZRF1 decreases the survival of estrogen-positive breast cancer patients more than estrogen-negative status patients. In the perspective of this, we evaluated the role ZRF1 in MCF-7 breast cancer cells and found that it's silencing by knockdown results in decreased cell proliferation as well as cell viability. Results also show that expression of ZRF1 is down regulated in the presence of estrogen-depleted conditions but independent of RAS/MEK as well as AKT axes. Moreover, the decrease in viability of MCF-7 cells was accompanied by induction of apoptosis and DNA damage, well-marked with upregulation of cleaved PARP and downregulation of BCL2 and H2AUbK119 levels. Furthermore, we also explored that knockdown of ZRF1 sensitises the effect of curcumin, observed with decrease in cell viability and dropping of IC50 value from 25 to 15 μM. This investigation thus shed a new light on the role on ZRF1 in breast cancer cells and hence can be exploited to design better therapeutic intervention. |
| Databáze: | OpenAIRE |
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