Improvement of Fusel Alcohol Production by Engineering of the Yeast Branched-Chain Amino Acid Aminotransaminase

Autor:	Jirasin Koonthongkaew, Nontawat Ploysongsri, Yoichi Toyokawa, Vithaya Ruangpornvisuti, Hiroshi Takagi
Rok vydání:	2022
Předmět:	Mitochondrial Proteins Saccharomyces cerevisiae Proteins Ethanol Ecology Saccharomyces cerevisiae Applied Microbiology and Biotechnology Amino Acids Branched-Chain Transaminases Food Science Biotechnology
Zdroj:	Applied and Environmental Microbiology. 88
ISSN:	1098-5336 0099-2240
Popis:	Branched-chain higher alcohols (BCHAs), or fusel alcohols, including isobutanol, isoamyl alcohol, and active amyl alcohol, are useful compounds in several industries. The yeast Saccharomyces cerevisiae can synthesize these compounds via the metabolic pathways of branched-chain amino acids (BCAAs). Branched-chain amino acid aminotransaminases (BCATs) are the key enzymes for BCHA production via the Ehrlich pathway of BCAAs. BCATs catalyze a bidirectional transamination reaction between branched-chain α-keto acids (BCKAs) and BCAAs. In S. cerevisiae, there are two BCAT isoforms, Bat1 and Bat2, which are encoded by the genes
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5a1de67156ab6bd9d5bc8c5e39a179e https://doi.org/10.1128/aem.00557-22 Zobrazit plný text záznamu