Insulin secretion by pancreas of athymic mice injected with peripheral mononuclear cells from insulin-dependent diabetic patients
| Autor: | L. Fabiano de Bruno, G. Gagliardi, J. C. Basabe, C.J. Quintans, Mabel Arata, W. M. Goncalvez Volpini |
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| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty endocrine system diseases Adolescent Cell Transplantation Endocrinology Diabetes and Metabolism medicine.medical_treatment Mitomycin Radioimmunoassay Mice Nude Biology Peripheral blood mononuclear cell Monocytes Islets of Langerhans Mice Endocrinology Immune system Internal medicine Immunopathology Insulin Secretion medicine Concanavalin A Animals Humans Insulin Rats Wistar Child Pancreas Pancreatic hormone Cells Cultured Autoimmune disease Mice Inbred BALB C Antibiotics Antineoplastic nutritional and metabolic diseases medicine.disease Rats medicine.anatomical_structure Diabetes Mellitus Type 1 Basal (medicine) Child Preschool Female |
| Zdroj: | Metabolism: clinical and experimental. 44(11) |
| ISSN: | 0026-0495 |
| Popis: | We studied the effect of peripheral blood mononuclear cells (PBMNC) from insulin-dependent diabetic (IDDM) children on the insulin secretion pattern of the pancreas from recipient athymic mice. PBMNC from healthy controls or IDDM patients in different stages of disease were injected into athymic mice. PBMNC from newly diagnosed IDDM children elicited basal nonfasting hyperglycemia and in vitro inhibition of the first and second phases of glucose-stimulated insulin secretion in recipient mice. Animals injected with cells from chronically IDDM children showed normoglycemia, abnormal tolerance to glucose, and inhibition of first-phase insulin secretion. Mitomycin C treatment of MNC from IDDM patients abolished insulin secretion inhibition in recipient mice. PBMNC from newly diagnosed and chronically IDDM patients showed positive anti-β-cell cellular immune aggression. Mice injected with cells from patients during the remission period showed normoglycemia and no alteration of insulin secretion patterns. When relapsed to their former clinical stage, injection of the cells significantly inhibited first-phase glucose-induced insulin secretion in recipients. PBMNC from newly diagnosed IDDM patients were found to migrate to the pancreas of recipient mice preferably as compared with cells from controls. Cells from chronically IDDM patients cultured with concanavalin A (Con A) increased insulin secretion inhibition; despite this, cells from children during the remission period cultured with Con A failed to modify insulin secretion in recipients. These results show that injection of PBMNC from diabetic patients leads to insulin secretion impairment in recipient mice pancreas, and provide a basis for the study of mechanisms involved in the onset and modulation of anti-β-cell cellular immune aggression induced by human PBMNC. |
| Databáze: | OpenAIRE |
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