Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand
| Autor: | Pakapak Chor Charoenying, Pathom Sawanpanyalert, Pranee Thawachsupa, Prasert Auewarakul, Pilaipan Puthavathana, Raweewan Khanyok, Rungrueng Kijphati, Kobporn Boonnak, Kantima Sangsiriwut, Phisanu Pooruk |
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| Rok vydání: | 2005 |
| Předmět: |
viruses
Molecular Sequence Data Orthomyxoviridae Neuraminidase Hemagglutinin Glycoproteins Influenza Virus medicine.disease_cause Antiviral Agents Genome H5N1 genetic structure Virus Disease Outbreaks Microbiology Viral Matrix Proteins Species Specificity Virology Drug Resistance Viral Influenza Human Amantadine medicine Influenza A virus Animals Humans Phylogeny Molecular Epidemiology Influenza A Virus H5N1 Subtype Molecular epidemiology biology Thailand biology.organism_classification Influenza A virus subtype H5N1 Influenza in Birds Mutation biology.protein RNA Viral Receptors Virus Chickens Sequence Alignment |
| Zdroj: | Journal of General Virology. 86:423-433 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.80368-0 |
| Popis: | The complete genomes of three human H5N1 influenza isolates were characterized, together with the haemagglutinin (HA) and neuraminidase (NA) genes from two additional human isolates and one chicken isolate. These six influenza isolates were obtained from four different provinces of Thailand during the avian influenza outbreak in Asia from late 2003 to May 2004. All six Thailand isolates contained multiple basic amino acids at the cleavage site in the HA gene. Amino acid residues at the receptor-binding site of the five human viruses were similar to those of the chicken virus and other H5N1 viruses from Hong Kong. The presence of amantadine resistance in the Thailand viruses isolated during this outbreak was suggested by a fixed mutation in M2 and confirmed by a phenotypic assay. All genomic segments of the Thailand viruses clustered with the recently described genotype Z. The Thailand viruses contained more avian-specific residues than the 1997 Hong Kong H5N1 viruses, suggesting that the virus may have adapted to allow a more efficient spread in avian species. |
| Databáze: | OpenAIRE |
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