Reversibility of experimental acute renal failure in rats: Assessment with USPIO-enhanced MR imaging
| Autor: | Elisabeth Schouman-Claeys, Jean Pierre Laissy, Hélène Beaufils, Sylvie Chillon, Ara Loshkajian, J. M. Idee, Soraya Benderbous |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Pathology medicine.medical_specialty Necrosis Iron medicine.medical_treatment Contrast Media Diatrizoate Nephrectomy Sensitivity and Specificity Rats Sprague-Dawley Iodinated contrast In vivo medicine Animals Radiology Nuclear Medicine and imaging Magnetite Nanoparticles Saline Observer Variation Analysis of Variance Kidney medicine.diagnostic_test business.industry Dextrans Oxides Magnetic resonance imaging Histology Acute Kidney Injury Magnetic Resonance Imaging Ferrosoferric Oxide Rats medicine.anatomical_structure Creatinine Injections Intravenous Linear Models medicine.symptom business Nuclear medicine medicine.drug |
| Zdroj: | Journal of Magnetic Resonance Imaging. 12:278-288 |
| ISSN: | 1522-2586 1053-1807 |
| DOI: | 10.1002/1522-2586(200008)12:2<278::aid-jmri10>3.0.co;2-v |
| Popis: | The purpose of this study was to evaluate the potential reversibility of kidney lesions in an experimental model of acute renal failure using ultra-small particles of iron oxide (USPIO)-enhanced magnetic resonance (MR) imaging. This study was conducted in 21 uninephrectomized rats using a model of iodinated contrast media-induced renal failure. Thirteen rats received selective intraarterial renal administration of diatrizoate (370 mg/ml) and were compared with two control groups, including six animals injected with saline and two noninjected animals. MR imaging was performed 28 hours, 8 days, and 22 days after the procedure. Each MR session included axial and coronal T1- and coronal T2-weighted images before and after intravenous administration of 60 μmol Fe/kg of USPIO. The rats were sacrificed immediately after the last MR session for pathologic evaluation. MR images were qualitatively and quantitatively interpreted with respect to pathologic data, and differences were statistically studied. At day 22, histology showed 4 severely diseased kidneys with focal areas of necrosis, 5 mildly diseased kidneys with tubular vacuolization, and 12 normal kidneys. On quantitative data, a high correlation between the percentage of negative enhancement and histologic data was observed (P < 0.05). Qualitative interpretation showed a sensitivity and specificity of USPIO-enhanced T2-weighted MR images of 88% and 91%, respectively. Follow-up enhancement curves showed a constant increase of intrarenal USPIO negative enhancement in normal kidneys between day 1 and day 22, whereas all severely involved kidneys displayed higher USPIO negative enhancement at day 1 without significant changes over time until day 22. USPIO may be useful for in vivo follow-up of the reversibility of experimentally induced iodinated contrast media renal impairment in animals. J. Magn. Reson. Imaging 2000;12:278–288. © 2000 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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