Increased Expression of a Disintegrin and Metalloprotease 8 in Allergic Rhinitis
| Autor: | Heung Man Lee, Tae Hoon Kim, Hyuk Choi, Seong Woong Choi, Sang Hag Lee, Seung Hoon Lee, Il Ho Park |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Rhinitis Allergic Perennial Mucous membrane of nose Inflammation Turbinates Western blot Disintegrin Animals Humans Immunology and Allergy Medicine Antigens Dermatophagoides Skin Tests Submucosal glands Metalloproteinase medicine.diagnostic_test biology Reverse Transcriptase Polymerase Chain Reaction business.industry Pyroglyphidae Membrane Proteins General Medicine Immunohistochemistry Up-Regulation ADAM Proteins Nasal Mucosa Otorhinolaryngology Immunology biology.protein Female medicine.symptom business ADAM8 |
| Zdroj: | American Journal of Rhinology & Allergy. 25:e107-e111 |
| ISSN: | 1945-8932 1945-8924 |
| DOI: | 10.2500/ajra.2011.25.3581 |
| Popis: | Background A disintegrin and metalloproteases (ADAMs) constitute a family of cell surface proteins containing disintegrin and metalloprotease domain, which associate features of adhesion molecules and proteases. ADAM8 is a member of the ADAM family and has recently been implicated to have a role in allergic lung inflammation. Therefore, our aims were to determine the expression of ADAM8 mRNA and protein and to localize ADAM8 protein in the nasal mucosa of patients with allergic rhinitis and control subjects. Methods Inferior turbinate mucosa samples were obtained from 20 patients with allergic rhinitis and 20 matched healthy normal controls. ADAM8 mRNA was extracted from the inferior turbinate mucosa, and then reverse transcription–polymerase chain reaction was performed. Western blot testing was used to analyze differences in the level of ADAM8 protein expression between patients with allergic rhinitis and normal controls, and the ADAM8 protein was localized with immunohistochemical staining. Results The level of expression of ADAM8 mRNA and protein in the nasal mucosa was significantly increased in patients with allergic rhinitis compared with normal controls. ADAM8 protein was expressed in the epithelium, infiltrating inflammatory cells, and submucosal glands. Conclusion ADAM8 is expressed in human nasal mucosa and is increased in patients with allergic rhinitis. These results suggest a possible contribution for ADAM8 in chronic inflammation of the nasal mucosa in patients with allergic rhinitis. |
| Databáze: | OpenAIRE |
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