Cryopreservation of Paramecium bursaria Chlorella Virus-1 during an active infection cycle of its host
| Autor: | James L. Van Etten, Steven W. Wilhelm, Alyssa N. Alsante, Samantha R. Coy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Microbiological culture Cell Membranes Plant Science Toxicology Pathology and Laboratory Medicine Biochemistry 01 natural sciences Cryopreservation Medicine and Health Sciences Phycodnaviridae Plant Hormones Psychrophile Infectivity 0303 health sciences Multidisciplinary Organic Compounds Plant Biochemistry Physics Eukaryota Plants Condensed Matter Physics Chemistry Virion assembly Physical Sciences Medicine Cellular Structures and Organelles Chlorella vulgaris Phase Transitions Research Article Algae Cryoprotectant Science Specimen Preservation Thawing Biology Research and Analysis Methods Microbiology Virus Cryobiology Ethylene 03 medical and health sciences Virology Plant Diseases 030304 developmental biology Toxicity 030306 microbiology Host (biology) 010604 marine biology & hydrobiology Organic Chemistry Organisms Chemical Compounds Biology and Life Sciences Cell Biology Viral Replication Hormones Viral replication Specimen Preparation and Treatment |
| Zdroj: | PLoS ONE, Vol 14, Iss 3, p e0211755 (2019) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Best practices in laboratory culture management often include cryopreservation of microbiota, but this can be challenging with some virus particles. By preserving viral isolates researchers can mitigate genetic drift and laboratory-induced selection, thereby maintaining genetically consistent strains between experiments. To this end, we developed a method to cryopreserve the model, green-alga infecting virus,Paramecium bursaria Chlorella virus 1(PBCV-1). We explored cryotolerance of the infectivity of this virus particle, whereby freezing without cryoprotectants was found to maintain the highest infectivity (~2.5%). We then assessed the cryopreservation potential of PBCV-1 during an active infection cycle in itsChlorella variabilisNC64A host, and found that virus survivorship was highest (69.5 ± 16.5 %) when the infected host is cryopreserved during mid-late stages of infection (i.e., coinciding with virion assembly). The most optimal condition for cryopreservation was observed at 240 minutes post-infection. Overall, utilizing the cell as a vehicle for viral cryopreservation resulted in 24.9 – 30.1 fold increases in PBCV-1 survival based on 95% confidence intervals of frozen virus particles and virus cryopreserved at 240 minutes post-infection. Given that cryoprotectants are often naturally produced by psychrophilic organisms, we suspect that cryopreservation of infected hosts may be a reliable mechanism for virus persistence in non-growth permitting circumstances in the environment, such as ancient permafrosts. |
| Databáze: | OpenAIRE |
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