Bioactive Constituents of Indigofera spicata
| Autor: | A. Douglas Kinghorn, Daniel D. Lantvit, Djaja Djendoel Soejarto, Lynette Bueno Pérez, Jie Li, Hee Byung Chai, Li Pan, Steven M. Swanson, David M. Lucas, Tran Ngoc Ninh |
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| Rok vydání: | 2013 |
| Předmět: |
Chalcone
Pharmaceutical Science Biology Reductase Article Analytical Chemistry Mice chemistry.chemical_compound In vivo Drug Discovery Animals Humans Bioassay Cytotoxic T cell Nuclear Magnetic Resonance Biomolecular Pharmacology Molecular Structure Organic Chemistry biology.organism_classification Antineoplastic Agents Phytogenic Indigofera Quinone Vietnam Complementary and alternative medicine Biochemistry chemistry Cell culture Flavanones Molecular Medicine Female Drug Screening Assays Antitumor HT29 Cells |
| Zdroj: | Journal of Natural Products. 76:1498-1504 |
| ISSN: | 1520-6025 0163-3864 |
| DOI: | 10.1021/np400567c |
| Popis: | Four new flavanones, designated as (+)-5″-deacetylpurpurin (1), (+)-5-methoxypurpurin (2), (2S)-2,3-dihydrotephroglabrin (3), and (2S)-2,3-dihydrotephroapollin C (4), together with two known flavanones (5 and 6), three known rotenoids (7-9), and one known chalcone (10) were isolated from a chloroform-soluble partition of a methanol extract from the combined flowers, fruits, leaves, and twigs of Indigofera spicata, collected in Vietnam. The compounds were obtained by bioactivity-guided isolation using the HT-29 human colon cancer, 697 human acute lymphoblastic leukemia, and Raji human Burkitt's lymphoma cell lines. The structures of 1-4 were established by extensive 1D- and 2D-NMR experiments, and the absolute configurations were determined by the measurement of specific rotations and CD spectra. The cytotoxic activities of the isolated compounds were tested against the HT-29, 697, Raji, and CCD-112CoN human normal colon cells. Also, the quinone reductase induction activities of the isolates were determined using the Hepa 1c1c7 murine hepatoma cell line. In addition, cis-(6aβ,12aβ)-hydroxyrotenone (7) was evaluated in an in vivo hollow fiber bioassay using HT-29, MCF-7 human breast cancer, and MDA-MB-435 human melanoma cells. |
| Databáze: | OpenAIRE |
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