Association between p21 Ser31Arg polymorphism and the development of cervical lesion in women infected with high risk HPV

Autor: Sergio Crovella, Fernanda Cristina Bezerra Leite, Sandra de Andrade Heráclio, Hildson Dornelas Angelo, Erinaldo Ubirajara Damasceno dos Santos, Paulo Roberto Eleutério de Souza, Celso P. de Melo, Maria Leonor Alves Maia, Gessica Dayane Cordeiro de Lima, Micheline Ramos de Oliveira
Přispěvatelé: Lima, Géssica, Santos, Erinaldo, Angelo, Hildson, Oliveira, Micheline, Heráclio, Sandra, Leite, Fernanda, de Melo, Celso, Crovella, Sergio, Maia, Maria, Souza, Paulo
Rok vydání: 2015
Předmět:
0301 basic medicine
Uterine Cervical Neoplasm
Pathology
Uterine Cervical Neoplasms
Gastroenterology
Polymerase Chain Reaction
law.invention
0302 clinical medicine
law
Genotype
Young adult
Polymerase chain reaction
Cervical cancer
General Medicine
Single Nucleotide
Middle Aged
Cell cycle
HPV
SNPs
Adolescent
Adult
Aged
Brazil
Cervical Intraepithelial Neoplasia
Cyclin-Dependent Kinase Inhibitor p21
Cyclin-Dependent Kinase Inhibitor p27
Female
Genetic Predisposition to Disease
Humans
Papillomavirus Infections
Young Adult
Polymorphism
Restriction Fragment Length
Polymorphism
Single Nucleotide
030220 oncology & carcinogenesis
Human
medicine.medical_specialty
SNP
Single-nucleotide polymorphism
Biology
Cervical intraepithelial neoplasia
03 medical and health sciences
Internal medicine
medicine
Allele
Polymorphism
Papillomavirus Infection
medicine.disease
Uterine Cervical Dysplasia
030104 developmental biology
Restriction Fragment Length
Zdroj: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 37(8)
ISSN: 1423-0380
Popis: Infection by high-risk human papillomavirus (HR-HPV) and single nucleotide polymorphism (SNP) in genes involved in cell cycle control, as p21 and p27, are important factors in the development of different types of human cancers. This study aims at investigating whether both the p21 Ser31Arg and p27 V109G polymorphisms are associated with susceptibility to the development of cervical lesions in women HR-HPV positive. We analyzed 132 women HPV positive and with cervical lesions or CC and 154 healthy control (HPV negative and without cervical lesions). p21 Ser31Arg and p27 V109G polymorphisms were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and sequencing. The p21 31Arg allele was associated with susceptibility for the development of cervical lesions (P* = 0.0009), while p27 V109G polymorphism showed no significant differences for this association (P* = 0.89). However, the combined effect of the polymorphisms showed that the presence of the CC genotype (SNP p21 Ser31Arg) conferred protection for the development of cervical lesions (OR = 0.39). p21 Ser31Arg and p27 V109G polymorphisms were not associated with the grade of cervical lesions (CINI, CINII, and CINIII) or CC (P* > 0.05). The HR-HPV more frequent in this study were of 16 (57.6 %) and 18 (37.1 %) types; however, no association was observed when both polymorphisms and risk factors analyzed were compared (P* > 0.05). Our findings suggest a possible association between p21 Ser31tabArg polymorphism and susceptibility to the development of cervical lesions in women from Pernambuco. Brazil.
Databáze: OpenAIRE
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