Chlorogenic acid enhances abdominal skin flap survival based on epigastric artery in nondiabetic and diabetic rats

Autor: Naciye Isbil Buyukcoskun, Musa Ozgur Ozyigit, Nilufer Cinkilic, Deniz Bagdas, Betul Cam Etoz, Mine Sibel Gurun, Kasim Ozluk, Zulfiye Gul, Sevda Inan
Přispěvatelé: Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi., Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Eczacılık Anabilim Dalı., Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı., Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Bağdaş, Deniz, Etöz, Betül Çam, Gül, Zülfiye, Özyiğit, Musa Özgür, Çinkılıç, Nilüfer, İnan, Sevda, Büyükcoşkun, Naciye İsbil, Özlük, Kasım, Gürün, Mine Sibel, AAF-9939-2020, AAH-5296-2021, E-3364-2018, AAR-6478-2021, AAH-1692-2021, AAG-8716-2019, AAH-2873-2021
Jazyk: angličtina
Rok vydání: 2014
Předmět:
0301 basic medicine
Rats
wistar
Antioxidant
Physiology
medicine.medical_treatment
Assay
Wistar rat
Antioxidants
chemistry.chemical_compound
Epigastric artery
0302 clinical medicine
Pathology
Diabetes mellitus
experimental
integumentary system
Abdominal skin
Flow
Experimental diabetes mellitus
Diabetes
Chlorogenic acid
Intraarterial drug administration
Flap survival
Flaps (Control Surfaces)
Perforator Flap
Reperfusion Injury
030220 oncology & carcinogenesis
endocrine system
medicine.medical_specialty
Wound healing
03 medical and health sciences
Necrosis
Abdominal wall
Injections
intra-arterial
Phenethyl ester
medicine
Animals
Drug effects
business.industry
Animal
Vascularization
Epigastric arteries
Surgery
Rats
Biological marker
030104 developmental biology
Metabolism
chemistry
Surgical flaps
Oxidative stress
Rat
business
Biomarkers
Model
Popis: Previous studies showed that chlorogenic acid (CGA) accelerates wound healing via its antioxidant activity. We aimed to investigate the effect of CGA in an experimental epigastric abdominal skin flap model in nondiabetic and diabetic rats. Rats were firstly divided into 2 groups: nondiabetic and diabetic. Diabetes was induced by streptozotocin. Then, 4 subgroups were created for each group: vehicle as well as 0.2 mg/0.5 mL, 1 mg/0.5 mL, and 5 mg/0.5 mL CGA treatments. Right epigastric artery-based abdominal skin flaps were elevated and sutured back into their original position. Chlorogenic acid or vehicle was injected once into the femoral arteries by leaving the epigastric artery as the single artery feeding the flaps during the injection. On postoperative day 7, flap survivals were evaluated, and the rats were killed. Distal flap tissues were collected for histopathological and biochemical assays. Chlorogenic acid showed greater flap survival in both nondiabetic and diabetic rats. Capillary density was increased, and necrosis was reduced in the CGA-treated rats. Chlorogenic acid decreased malondialdehyde levels as well as increased reduced glutathione and superoxide dismutase levels in the flap tissues. This study showed that CGA significantly improved flap survival by its antioxidant activities with intra-arterial local injections.
Databáze: OpenAIRE
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