Identification of the Ricin-B-Lectin LdRBLk in the Colorado Potato Beetle and an Analysis of Its Expression in Response to Fungal Infections
| Autor: | M. V. Tyurin, Ulyana N. Rotskaya, Elena Kosman, Vadim Yu. Kryukov, Viktor V. Glupov |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
QH301-705.5 heat shock protein Beauveria bassiana Plant Science Article Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Heat shock protein C-type lectins Biology (General) Leptinotarsa Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences biology RCA Colorado potato beetle fungi domain pfam00652 Lectin biology.organism_classification attacin Fungicide Ricin chemistry antifungal peptide biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Journal of Fungi, Vol 7, Iss 364, p 364 (2021) Journal of Fungi Volume 7 Issue 5 |
| Popis: | Ricin-B-lectins (RBLs) have been identified in many groups of organisms, including coleopterans insects, particularly the Colorado potato beetle Leptinotarsa decemlineata (LdRBLs). We hypothesized that one of these LdRBLs (LdRBLk) may be involved in the immune response to fungal infections. We performed a theoretical analysis of the structure of this protein. Additionally, the expression levels of the LdRBlk gene were measured in L. decemlineata in response to infections with the fungi Metarhizium robertsii and Beauveria bassiana. The expression levels of LdRBlk in the L. decemlineata cuticle and fat body were increased in response to both infections. The induction of LdRBlk expression was dependent on the susceptibility of larvae to the fungi. Upregulation of the LdRBlk gene was also observed in response to other stresses, particularly thermal burns. Elevation of LdRBlk expression was frequently observed to be correlated with the expression of the antimicrobial peptide attacin but was not correlated with hsp90 regulation. Commercially available β-lectin of ricin from Ricinuscommunis was observed to inhibit the germination of conidia of the fungi. We suggest that LdRBLk is involved in antifungal immune responses in the Colorado potato beetle, either exerting fungicidal properties directly or acting as a modulator of the immune response. |
| Databáze: | OpenAIRE |
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