Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies
| Autor: | Aytül Gül, Aykut Ozdarendeli, Philip L. Felgner, Huw Davies, Nazif Elaldi, Mert Döşkaya, Muhammet Karakavuk, Aysu Değirmenci Döşkaya, Adnan Yüksel Gürüz, Esra Atalay Şahar, Hüseyin Can, Gizem Örs Kumoğlu, Sultan Gulce-Iz |
|---|---|
| Přispěvatelé: | Ege Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Science 030106 microbiology Enzyme-Linked Immunosorbent Assay Antibodies Viral Sensitivity and Specificity Virus Article Serology law.invention 03 medical and health sciences Antigen law Interquartile range Medicine Humans Multidisciplinary biology Molecular medicine business.industry Reproducibility of Results Diagnostic markers Hemorrhagic fever virus Prognosis Virology Recombinant Proteins 030104 developmental biology [No Keyword] Viral infection Immunoglobulin G Hemorrhagic Fever Virus Crimean-Congo Recombinant DNA biology.protein Hemorrhagic Fever Crimean Antibody business Crimean Congo hemorrhagic fever virus Biomarkers |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral infection caused by Crimean-Congo hemorrhagic fever virus (CCHFV). Serological screening of CCHF is important and current ELISA use antigens prepared from virus which is expensive due to requirement of high bio-containment facilities. in this study, we aimed to develop a new recombinant ELISA. For this purpose, CCHFV genome were expressed as 13 proteins in E. coli and among them abundantly purified recombinant Nucleocapsid protein (rNP) and Mucin-like variable domain (rMLD) were used as antigen in ELISA (Rec-ELISA). Rec-ELISA using rNP, rMLD and a combination of both (rNP/rMLD) were probed with acute (n=64; collected between days 1 and 7 after onset of symptoms), convalescent (n=35; collected 8 days after onset of symptoms), consecutive sera (n=25) of confirmed CCHF cases and control sera (n=43). The sensitivity and specificity of Rec-ELISA using rNP/rMLD were 73% and 98% in acute cases and 97% and 98% in convalescent cases. The median interquartile absorbance value to discriminate the acute and convalescent phases of CCHF was significantly higher with ELISA using rNP/rMLD (P0.05) and rMLD (P=0.001). These results indicate that the Rec-ELISA using rNP/rMLD may be very useful to diagnose convalescent CCHF cases especially in field studies. Turkish Scientific and Technological Research Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) The authors would like to acknowledge Turkish Scientific and Technological Research Council (TUBITAK) for supporting Sultan Gulce-z by a 2219 post-doctoral research fellowship programme to conduct part of the studies at Department of Infectious Diseases, University of California, Irvine. |
| Databáze: | OpenAIRE |
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