Voltage gated K+ channel expression in arteries of Wistar-Kyoto and spontaneously hypertensive rats
| Autor: | Samantha J. Fromme, Kimberly Folander, Robert H. Cox, Richard Swanson |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Tail medicine.medical_specialty Patch-Clamp Techniques Myogenic contraction Gene Expression CHO Cells Kidney Rats Inbred WKY Monocytes Kv1.5 Potassium Channel Cricetulus Shab Potassium Channels Thoracic Arteries Antibody Specificity medicine.artery Internal medicine Cricetinae Rats Inbred SHR Gene expression Internal Medicine medicine Kv1.2 Potassium Channel Myocyte Animals Humans cardiovascular diseases Patch clamp Mesenteric arteries Aorta Voltage-gated ion channel business.industry Potassium channel Mesenteric Arteries Rats medicine.anatomical_structure Endocrinology Potassium Channels Voltage-Gated Hypertension business |
| Zdroj: | American journal of hypertension. 21(2) |
| ISSN: | 0895-7061 |
| Popis: | Background We have previously demonstrated differences in the gene expression of voltage-gated K v1.X channel alpha-subunits in arteries from Wistar-Kyoto rats (WKYs) and spontaneously hypertensive rats (SHRs). The purpose of this study was to test the hypothesis that these differences are also present at the protein level. Methods Proteins were isolated from the aorta, mesenteric (MAs) and tail arteries (TAs) of 12- to 15-week-old male WKY and SHR, and analyzed by immunoblotting. K(v) currents were recorded from MA myocytes by patch clamp methods. Results Expression of Kv1.2, Kv1.5, and Kv2.1 was higher in MAs but was not different in aortas of SHRs as compared to WKYs. In the TA, expression of Kv1.2 and Kv1.5 was higher while that of Kv2.1 was lower in SHR compared to WKY. In the MA, the larger expression of an 80 kDa species of Kv1.2 in SHRs was associated with a lower expression of a 60 kDa species. Kv2.1 gene expression was larger in MAs from SHRs but not different in TAs. K(v) currents associated with Kv1.X and Kv2.1 channels were both larger in MA myocytes from SHRs but less than expected based upon differences in K(v) alpha-subunit protein expression. Conclusions For the MA, K(v) protein expression and current components between WKYs and SHRs were qualitatively consistent, but differences in gene and protein expression were not closely correlated. The higher expression of K(v) subunits in small mesenteric arteries (SMAs) of SHR would tend to maintain normal myogenic activity and vasoconstrictor reserve, and could be viewed as a form of homeostatic remodeling. |
| Databáze: | OpenAIRE |
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