Growth factor-mediated hyper-elongation of glycosaminoglycan chains on biglycan requires transcription and translation
| Autor: | Mandy L. Ballinger, Sundy N Y Yang, Peter J. Little, Micah L Burch, Robel Getachew, Narin Osman |
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| Rok vydání: | 2009 |
| Předmět: |
Platelet-derived growth factor
Time Factors Transcription Genetic Physiology medicine.medical_treatment Biology Cycloheximide Sulfur Radioisotopes Muscle Smooth Vascular chemistry.chemical_compound Epidermal growth factor Transforming Growth Factor beta Physiology (medical) Biglycan medicine Humans Cells Cultured Glycosaminoglycans Platelet-Derived Growth Factor Protein Synthesis Inhibitors Extracellular Matrix Proteins Dose-Response Relationship Drug Epidermal Growth Factor Growth factor Thrombin General Medicine Transforming growth factor beta Cell biology chemistry Biochemistry Proteoglycan biology.protein Dactinomycin Intercellular Signaling Peptides and Proteins Proteoglycans Platelet-derived growth factor receptor |
| Zdroj: | Archives of physiology and biochemistry. 115(3) |
| ISSN: | 1744-4160 |
| Popis: | The mechanism through which growth factors cause glycosaminoglycan (GAG) hyper-elongation is unclear. We have investigated the role of transcription and translation on the GAG hyper-elongation effect of platelet-derived growth factor (PDGF) in human vascular smooth muscle cells (VSMCs). To determine if the response involves specific signalling pathways or the process of GAG hyper-elongation we have also investigated the effects of epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta) and thrombin. We report that both actinomycin D and cycloheximide completely abolished the ability of PDGF to stimulate radiosulphate incorporation and GAG elongation into secreted proteoglycans, and to increase the size of xyloside GAGs. Blocking de novo protein synthesis completely prevented the action of all growth factors tested to elongate GAG chains. These results lay a foundation for further investigation into the genes and proteins implicated in this response. |
| Databáze: | OpenAIRE |
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